Distribution of tubulointerstitial nephritis antigen-like 1 and structural matrix proteins in mouse embryos during preimplantation development in vivo and in vitro.
Distribution of tubulointerstitial nephritis antigen-like 1 and structural matrix proteins in mouse embryos during preimplantation development in vivo and in vitro.
复制标题
体内和体外植入前发育过程中小鼠胚胎中肾小管间质性肾炎抗原样 1 和结构基质蛋白的分布。
DOI：
10.1017/s0967199412000469
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发表时间：
2014
期刊：
影响因子：
1.7
通讯作者：
Matsumotoら
中科院分区：
文献类型：
--
作者：
Sakurai;Matsumotoら
肾小管间质性肾炎抗原样蛋白1（TINAGL 1）是一种新型的基质细胞蛋白，与结构基质蛋白相互作用，促进细胞粘附和扩散。我们以前曾报道过TINAGL 1在小鼠囊胚滋养外胚层（TE）的独特定位。使用炔雌醇处理的延迟着床模型，在雌激素处理后，发现TINAGL 1在有发育能力的囊胚中上调。此外，TINAGL 1和细胞外基质（ECM）蛋白层粘连蛋白1的共定位检测在胚胎天6.5和7.5的Reichert膜。虽然这些数据表明TINAGL 1在植入后胚胎发育中的作用，但其与植入前发育过程中其他ECM蛋白的相关性尚不清楚。在这项研究中，我们研究了TINAGL 1的表达及其与其他ECM蛋白纤连蛋白（FN）和IV型胶原（ColIV）在体内发育的植入前胚胎，特别是在囊胚阶段的详细。TINAGL 1、FN和ColIV的定位相似。在1- 8细胞胚胎中，它们表达于卵裂球的细胞质中，在桑椹胚中，它们定位于外细胞。FN和ColIV主要表达于细胞外表面。在囊胚中，FN和ColIV分布在TE的细胞质中，但是，就在植入之前，它们变得独特地定位于TE的囊胚腔表面。在体外受精（IVF）囊胚，表达水平的TINAGL 1和FN低于在体内囊胚。这些结果表明，在植入前发育过程中，TINAGL 1可能参与了结构基质蛋白的作用，其在囊胚中的表达可能受到体外培养的影响。
Tubulointerstitial nephritis antigen-like 1 (TINAGL1) is a novel matricellular protein that interacts with structural matrix proteins and promotes cell adhesion and spreading. We have previously reported unique localization of TINAGL1 to the trophectoderm (TE) of mouse blastocysts. TINAGL1 was found to be upregulated in implantation-competent blastocysts after estrogen treatment using progesterone-treated delayed-implantation models. Moreover, colocalization of TINAGL1 and extracellular matrix (ECM) protein laminin 1 was detected in the Reichert membrane on embryonic days 6.5 and 7.5. Although these data suggested a role for TINAGL1 in the embryo development at postimplantation, its relevance to other ECM proteins during preimplantation development is not clear. In this study, we examined the expression of TINAGL1 and its relevance to other ECM proteins fibronectin (FN) and collagen type IV (ColIV) during in vivo development of preimplantation embryos, particularly at blastocyst stage in detail. Localizations of TINAGL1, FN, and ColIV were similar. In 1-cell to 8-cell embryos, they were expressed in cytoplasm of blastomeres, and in morulae they were localized in the outer cells. FN and ColIV were expressed primarily on outer surface of the cells. In blastocysts, FN and ColIV were distributed in the cytoplasm of TE, but, just prior to implantation, they became localized uniquely to the blastocoelic surface of TE. In in vitro fertilized (IVF) blastocysts, expression levels of TINAGL1 and FN were lower than in in vivo blastocysts. These results suggest that, during preimplantation development, TINAGL1 may be involved in roles of structural matrix proteins, whose expression in blastocysts may be affected by in vitro culture.