Polyhydroxyalkanoate accumulation ability and associated microbial community in activated sludge-derived acetate-fed microbial cultures enriched under different temperature and pH conditions
Polyhydroxyalkanoate accumulation ability and associated microbial community in activated sludge-derived acetate-fed microbial cultures enriched under different temperature and pH conditions
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DOI：
10.1016/j.jbiosc.2017.09.008
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发表时间：
2018-03-01
影响因子：
2.8
通讯作者：
Sei, Kazunari
中科院分区：
文献类型：
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作者：
Inoue, Daisuke;Suzuki, Yuta;Sei, Kazunari
研究了富集过程中温度和pH值对富集培养物中聚羟基烷酸（PHA）富集能力和PHA富集微生物（PHAMOs）组成的影响。在不同温度（20 ° C、28 ° C和36 ° C）和pH（控制在7.2或不控制在7.2）条件下，在乙酸盐进料的序批式反应器中使用盛宴-饥饿方案从活性污泥中富集PHAMO。PHA的积累能力，这是在氮和磷缺乏24小时单批培养物中进行评估，大大提高了富集，无论温度和pH值。富集在20摄氏度或28摄氏度，没有pH值控制似乎最适合强PHA积累。通过靶向phaC基因（其编码I类和II类PHA酶）的克隆文库方法对PHAAMO组成的分析揭示，伯克霍尔德菌目是种子污泥中的优势PHAAMO，而红环菌目，特别是Azoarcus spp.和Thauera spp.，在不控制pH的情况下富集后，占优势，显示出较强的积累PHA的能力。结果表明，固氮线虫属（Azoarcus spp.）和Thauera spp.是基于盛宴-饥饿法的富集培养中的关键PHAMO，具有高PHA积累能力。(C)2017年，日本生物技术学会。All rights reserved.
The influence of temperature and pH during enrichment on the polyhydroxyalkanoate (PHA) accumulation ability and composition of PHA-accumulating microorganisms (PHAAMOs) in enrichment cultures was investigated. Enrichment of PHAAMOs from activated sludge was conducted in acetate-fed sequencing batch reactors using a feast-famine regime under different temperature (20 degrees C, 28 degrees C, and 36 degrees C) and pH (controlled at 7.2 or not) conditions. PHA accumulation ability, which was evaluated in nitrogen- and phosphorus-deficient 24-h single-batch cultures, was greatly enhanced by enrichment, irrespective of the temperature and pH. Enrichment at 20 degrees C or 28 degrees C and without pH control seemed most appropriate for strong PHA accumulation. Analyses of the PHAAMO composition by the clone library method targeting phaC genes, which encode the class I and II PHA synthases, revealed that Burkholderiales were the dominant PHAAMOs in the seed sludge, while Rhodocyclales, specifically Azoarcus spp. and Thauera spp., were dominant after enrichment without pH control, showing a strong ability to accumulate PHA. The results indicated that Azoarcus spp. and Thauera spp. are key PHAAMOs in an enrichment culture based on the feast-famine method, with high PHA accumulation ability. (C) 2017, The Society for Biotechnology, Japan. All rights reserved.