An enzyme-free ultrasensitive electrochemical immunosensor for calprotectin detection based on PtNi nanoparticles functionalized 2D Cu-metal organic framework nanosheets
An enzyme-free ultrasensitive electrochemical immunosensor for calprotectin detection based on PtNi nanoparticles functionalized 2D Cu-metal organic framework nanosheets
复制标题
基于 PtNi 纳米颗粒功能化二维铜金属有机骨架纳米片的无酶超灵敏电化学免疫传感器，用于检测钙卫蛋白
DOI：
10.1016/j.snb.2020.127687
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发表时间：
2020-04-01
影响因子：
8.4
通讯作者：
Xia, Qianfeng
中科院分区：
文献类型：
--
作者：
Dong, Lanlan;Yin, Li;Xia, Qianfeng
In this work, a novel high electro-catalytic material was synthesized using PtNi nanospheres to functionalize two-dimensional (2D) ultrathin Cu-TCPP(Fe) nanosheets (PtNi@Cu-TCPP(Fe)), and was further applied to develop an enzyme-free electrochemical immunosensor for the ultrasensitive determination of calprotectin (CALP). The bimetallic Cu-TCPP(Fe) nanosheets with large surface area and massive accessible active sites permitted multiple PtNi to attach their surface, which not only enhanced the catalytic ability and conductivity for nonenzymatic amplification but also provided the modified active sites for antibody immobilization as signal labels. Upon the dual electro-catalytic activity of Cu-TCPP(Fe) and PtNi towards H2O2 reduction for signal amplification, this proposed sandwich CALP immunosensor exhibited a wide linear range of 200 fg mL(-1)similar to 50 ng mL(-1) and a low detection limit of 137.7 fg mL(-1). The functionalized MOF nanosheets-based biosensing method offered an enzyme-free signal amplification strategy, which provided great promise for further bioanalysis and clinical study.
