Selection and identification of single domain antibody fragments from camel heavy-chain antibodies
Selection and identification of single domain antibody fragments from camel heavy-chain antibodies
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DOI：
10.1016/s0014-5793(97)01062-4
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发表时间：
1997-09-15
期刊：
影响因子：
3.5
通讯作者：
Muyldermans, S
中科院分区：
文献类型：
--
作者：
Ghahroudi, MA;Desmyter, A;Muyldermans, S
骆驼科动物中天然存在缺乏轻链的功能性重链γ-免疫球蛋白。我们现在展示了免疫单峰骆驼的可行性，克隆其重链抗体的可变结构域的库并淘选，从而成功鉴定最小尺寸的抗原结合物。这种方法可以被视为获得具有有利特性和有价值前景的小结合剂的一般途径，作为模块化构建块来制造多特异性或多功能嵌合蛋白。(C)1997年欧洲生物化学学会联合会。
Functional heavy-chain gamma-immunoglobulins lacking light chains occur naturally in Camelidae, We now show the feasibility of immunising a dromedary cloning the repertoire of the variable domains of its heavy-chain antibodies and panning, leading to the successful identification of minimum sized antigen binders, The recombinant binders are expressed web in E. coli, extremely stable, highly soluble, and react specifically and with high affinity to the antigens, This approach can be viewed as a general route to obtain small binders with favourable characteristics and valuable perspectives as modular building blocks to manufacture multispecific or multifunctional chimaeric proteins. (C) 1997 Federation of European Biochemical Societies.