The role of RNA and protein in ribosome function: a review of early reconstitution studies and prospects for future studies.
The role of RNA and protein in ribosome function: a review of early reconstitution studies and prospects for future studies.
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RNA 和蛋白质在核糖体功能中的作用：早期重建研究回顾和未来研究展望。
DOI：
10.1101/sqb.1987.052.01.075
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Nomura,M
中科院分区：
文献类型：
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作者：
Nomura,M
虽然近年来我们实验室的主要研究工作是核糖体成分的基因及其表达调控，但我同意写一篇文章回顾早期与核糖体结构和功能相关的工作，主要基于我们自己从20世纪60年代末到70年代初对核糖体重构的研究，以及对核糖体突变的一般研究。目前对核糖体结构和功能的研究重点是rRNA作为直接参与核糖体功能的可能成分，而不是早期的研究似乎集中在核糖体蛋白（r-蛋白）上。然而，应该注意的是，早期的工作者并没有忽视rnas的可能作用。事实上，人们不仅认识到了RNA的重要性，而且存在一些强有力的证据支持RNA发挥功能性作用的观点。然而，由于核糖体中有许多蛋白质，并且在早期技术上更容易解剖蛋白质，因此大多数研究都集中在蛋白质上。尽管有大量证据表明r-蛋白对组装很重要，但在这些早期研究中，r-蛋白在完成的核糖体中的功能作用仅对少数蛋白质有明确的确定。由于本卷的主题是催化功能的起源，审查旧的重构研究在这个时候可能仍然是相关的这一卷；它可能有助于评估r蛋白和rnas在当今核糖体中的相对作用，从而推测原始核糖体的性质及其向当今核糖体的进化。
Although the major research effort of our laboratory during recent years has been on the genes for ribosomal components and the regulation of their expression, I have agreed to write an article reviewing the earlier work related to ribosome structure and function, based mostly on our own research on ribosome reconstitution from the late 1960s to the early 1970s, as well as studies on ribosome mutations in general. The emphasis in current research on ribosome structure and function is on rRNA as the probable component directly involved in ribosome function, in contrast to the earlier studies that appeared to concentrate on ribosomal proteins (r-proteins). However, it should be noted that the earlier workers did not ignore possible roles of rRNAs. In fact, not only was the importance of rRNAs recognized, but some strong evidence existed supporting the idea that RNA played a functional role. However, because there are many proteins in the ribosomes and it was easier technically to dissect the proteins in those early days, most studies concentrated on the proteins. Although there was abundant evidence to indicate that r-proteins are important for assembly, functional roles of r-proteins in the finished ribosomes were clearly established only for a few proteins in these earlier studies. Since the main theme of this volume is the origin of catalytic function, reviewing the old reconstitution studies at this time may still be pertinent to this volume; it may be useful in assessing the relative roles of r-proteins and rRNAs in the present-day ribosomes, allowing speculation on the nature of the primitive ribosomes and their evolution to the present-day ribosomes.