The Application of Synchrotron Radiation‐Based Micro‐Computer Tomography in Biomineralization
The Application of Synchrotron Radiation‐Based Micro‐Computer Tomography in Biomineralization
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同步辐射微计算机断层扫描在生物矿化中的应用
DOI：
10.1002/9783527619443.ch21
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Epple M
中科院分区：
文献类型：
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作者：
Neues F;Beckmann F;Ziegler A;Epple M
基于同步辐射的微型计算机断层扫描（SRm CT）是一种无破坏技术，它提供了一种方便的方法来区分矿化组织和周围的有机组织。它的特殊性质使该方法成为研究生物矿化组织的理想方法。在计算机三维（3‐D）重建后进行虚拟切片，可使隐藏的内部结构可视化，并可避免“真实的生活”切割中发生的样本破坏。在这一章中，下面的方法本身的简要说明，其应用于斑马鱼（斑马鱼）和陆生甲壳类动物，常见的木虱，Porcellio scaber，提出。将讨论斑马鱼的牙齿和骨骼，以及Porcellio scaber的矿化外骨骼和胸骨碳酸钙沉积物。
Synchrotron radiation‐based micro‐computer tomography (SRm̈CT) is a destruction‐free technique which offers a convenient way to distinguish between mineralized tissues and the surrounding organic tissue. Its special properties make this method ideal for the study of biomineralized tissues. Virtual sectioning after computerized three‐dimensional (3‐D) reconstruction allows hidden internal structures to be visualized, and the advantage that destruction of the sample ‐ as occurs in “real‐life” cutting ‐ can be avoided. In this chapter, following a brief description of the method per se, its application to zebrafish (Danio rerio) and to a terrestrial crustacean, the common woodlouse, Porcellio scaber, are presented. The teeth and bone of Danio rerio, and the mineralized exoskeleton and sternal calcium carbonate deposits of Porcellio scaber, will be discussed.