A difunctional squarylium indocyanine dye distinguishes dead cells through diverse staining of the cell nuclei/membranes.
A difunctional squarylium indocyanine dye distinguishes dead cells through diverse staining of the cell nuclei/membranes.
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DOI：
10.1002/smll.201302920
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发表时间：
2014-04
期刊：
影响因子：
13.3
通讯作者：
Jie Li;Kunru Guo;Jie Shen;Wantai Yang;Meizhen Yin
中科院分区：
文献类型：
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作者：
Jie Li;Kunru Guo;Jie Shen;Wantai Yang;Meizhen Yin
功能化荧光染料在多细胞生物亚细胞器的特异性染色方面引起了人们极大的兴趣。合成了一种新型的含四个伯胺的纳米水溶性多功能方酸吲哚菁染料（D1）。该染料具有良好的光稳定性、无毒性和生物相容性。等温滴定量热法表明D1与DNA的亲和力高于D1与磷脂类似物的亲和力。圆二色谱分析表明，D1靶向DNA小沟和聚集成螺旋。由于染料和亚细胞器之间的独特亲和力，染料表现出双功能能力，以标记固定细胞/组织中的细胞核和活细胞/组织中的细胞膜。通过两种染色能力的组合，进一步探索染料作为区分活细胞/组织中凋亡细胞的特异性标记物。该研究为设计用于生命科学应用的新型多功能染料开辟了新途径。
Functionalized fluorescent dyes have attracted great interest for the specific staining of subcellular organelles in multicellular organisms. A novel nanometer-sized water-soluble multi-functional squarylium indocyanine dye (D1) that contains four primary amines is synthesized. The dye exhibits good photostability, non-toxicity and biocompatibility. Isothermal titration calorimetry demonstrates that an affinity between D1 and DNA is higher than that between D1 and analogue of phospholipids. Analysis of circular dichroism spectra indicates that D1 targets to the DNA minor groove and aggregates to a helix. Because of the distinct affinity between the dye and subcellular organelles, the dye exhibits difunctional abilities to label the cell nuclei in fixed cells/tissue and the cell membranes in live cells/tissue. By combination of the two staining capabilities, the dye is further explored as a specific marker to distinguish apoptotic cells in live cells/tissue. The research opens a new way to design novel multifunctional dyes for life science applications.