Erythrocyte preservation. VII. Acid-citrate-dextrose-inosine (ACDI) as a preservative for blood during storage at 4 degree C.
Erythrocyte preservation. VII. Acid-citrate-dextrose-inosine (ACDI) as a preservative for blood during storage at 4 degree C.
复制标题
红细胞保存。
DOI：
10.1172/jci103322
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发表时间：
1956
期刊：
影响因子：
--
通讯作者：
C. Finch
中科院分区：
文献类型：
--
作者：
B. W. Gabrio;D. M. Donohue;F. M. Huennekens;C. Finch
方法测定红细胞的磷酸盐分割、葡萄糖和渗透脆性的方法已在其他地方描述（3,6）。肌苷、腺苷、脱氧腺苷和腺嘌呤来源于Schwarz实验室；这项工作得到了美国原子能委员会和美国陆军军医总署办公室的研究资助。次黄嘌呤、鸟苷、黄嘌呤和核糖-5-磷酸。单硫酸腺苷和三硫酸腺苷是A. Hock博士赠送的，2,6 -二氨基嘌呤核苷是GB Brown博士赠送的。用几种溶剂体系在Whatman 1号滤纸上进行一维纸层析；已知标准的theRf值在表1中给出。嘌呤化合物的鉴定是通过在紫外光（Mineralite）下检查纸张完成的。斑点定位后，将物质从纸上洗脱，进行分光光度测定。通过247mua时的光吸收测量，计算了原液中肌苷的浓度
METHODSProcedures for the determination of phosphate parti-tion, glucose, and osmotic fragility of erythrocytes have been described elsewhere (3, 6). Inosine, adenosine, deoxyadenosine, and adenine were obtained from Schwarz Laboratories; guanine, xanthine, 1This work was supported by research grants from the United States Atomic Energy Commission and the Office of the Surgeon General, Department of the Army. hypoxanthine, guanosine, xanthosine, and ribose-5-phosphate from Nutritional Biochemicals Corporation. Adenosine mono-and tri-sulfate were gifts from Dr. A. Hock, and 2, 6-diaminopurine riboside from Dr. GB Brown. One-dimensional, paper chromatography on Whatman No. 1 filter paper was performed with several solvent systems; theRf values for known standards are given in Table I. Identification of the purine compounds was accomplished by inspection of the papers under ultra violet light (Mineralite). After location of the spots, the materials could be eluted from the paper and meas-ured spectrophotometrically. The concentration of inosine in stock solutions was cal-culated from light absorption measurements at 247 muA in