Two genes, atpC1 and atpC2, for the gamma subunit of Arabidopsis thaliana chloroplast ATP synthase.
Two genes, atpC1 and atpC2, for the gamma subunit of Arabidopsis thaliana chloroplast ATP synthase.
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发表时间：
1991-04
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通讯作者：
N. Inohara;A. Iwamoto;Y. Moriyama;S. Shimomura;M. Maeda;M. Futai
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作者：
N. Inohara;A. Iwamoto;Y. Moriyama;S. Shimomura;M. Maeda;M. Futai
拟南芥有两个编码叶绿体 ATP 合酶 γ 亚基的基因（atpC1、atpC2）。克隆并测序了 atpC1 和 atpC2。它们的阅读框内没有内含子，分别编码 373 和 386 个氨基酸残基的蛋白质，包括推定的转运序列（分别为 50 和 60 个氨基酸残基）。相比之下，菠菜γ亚基基因在阅读框中有两个内含子。拟南芥两个基因编码的成熟序列（atpC1，323个残基；atpC2，326个残基）与菠菜的成熟序列同源（J. Miki, M. Maeda, Y. Mukohata, and M. Futai (1988) FEBS Lett. 232, 221-226）：atpC1编码的γ亚基的同源性其中atpC2和atpC2为72%，atpC1和菠菜cDNA编码的亚基为84%，atpC2和菠菜cDNA编码的蛋白质为71%。与菠菜亚基一样，这两个基因编码的伽马亚基具有线粒体或细菌亚基中未发现的独特调节域。在光照植物中检测到对应于atpC1（1.5kb）和atpC2（2.5kb）的Poly(A)+ mRNA，前者的量至少是后者的140倍。在暗适应植物中未发现 atpC1 mRNA。通过凝胶位移测定检测特异性结合到 atpC1 上游区域的核蛋白，并且其结合显示出被编码核酮糖-1，5-二磷酸羧化酶小亚基的基因的 GT-1 元件抑制，该基因在光照下表达（P. J. Green、S. A. Kay 和 N. H. Chau (1987) EMBO J. 6, 2543-2549)。与这些发现一致的是，在光照植物中免疫化学检测到了γ亚基数量的增加。
Arabidopsis thaliana has two genes (atpC1, atpC2) coding for gamma subunits of chloroplast ATP synthase. The atpC1 and atpC2 were cloned and sequenced. They had no introns within the reading frames and coded for proteins of 373 and 386 amino acid residues, respectively, including putative transit sequences (50 and 60 amino acid residues, respectively). In contrast, the spinach gamma subunit gene had two introns within the reading frame. The mature sequences coded by the two genes of A. thaliana (atpC1, 323 residues; atpC2, 326 residues) were homologous with that of spinach (J. Miki, M. Maeda, Y. Mukohata, and M. Futai (1988) FEBS Lett. 232, 221-226): the homologies of gamma subunits coded by atpC1 and atpC2 were 72%, those of the subunits coded by atpC1 and spinach cDNA were 84%, and those of the proteins coded by atpC2 and spinach cDNA were 71%. Like the spinach subunit, the gamma subunits coded by the two genes had unique regulatory domains not found in mitochondrial or bacterial subunits. Poly(A)+ mRNAs corresponding to atpC1 (1.5 kilobases) and atpC2 (2.5 kilobases) were detected in illuminated plants, the amount of the former being at least 140 times that of the latter. The atpC1 mRNA was not found in dark-adapted plants. Nuclear protein(s) specifically bound to the upstream region of atpC1 was detected by gel shift assay and its binding was shown to be inhibited by the GT-1 element of the gene encoding the ribulose-1,5-bisphosphate carboxylase small subunit, which is expressed under illumination (P. J. Green, S. A. Kay, and N. H. Chau (1987) EMBO J. 6, 2543-2549). Consistent with these findings, an increased amount of the gamma subunit was detected immunochemically in illuminated plants.