Bioinspired and Biomimetic AgNPs/Gentamicin-Embedded Silk Fibroin Coatings for Robust Antibacterial and Osteogenetic Applications
Bioinspired and Biomimetic AgNPs/Gentamicin-Embedded Silk Fibroin Coatings for Robust Antibacterial and Osteogenetic Applications
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仿生 AgNPs/庆大霉素包埋丝素蛋白涂层，用于强大的抗菌和成骨应用
DOI：
10.1021/acsami.7b06757
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发表时间：
2017
影响因子：
9.5
通讯作者：
Zhenie Yufeng
中科院分区：
文献类型：
--
作者：
Zhou Wenhao;Jia Zhaojun;Xiong Pan;Yan Jianglong;Li Yangyang;Li Ming;Cheng Yan;Zhenie Yufeng
随着骨科钛植入物需求的逐步增加，需要解决限制植入物应用的两个主要并发症之间的平衡：缺乏骨组织整合和生物医学器械相关感染（BAI），其中多重耐药细菌的出现使其变得更糟。值得注意的是，银纳米颗粒（AgNPs）和一种抗生素的组合可以协同抑制细菌生长，其中低浓度的AgNPs已经被证实促进成骨细胞的增殖和成骨。在这项工作中，我们建立了银纳米粒子/庆大霉素（Gen）嵌入丝素蛋白（SF）为基础的仿生涂层骨科钛通过一个简单的浸渍-干燥循环过程中，并与聚多巴胺（PD）的援助。在紫外光照射下，银离子被SF还原为AgNPs，并通过透射电子显微镜（TEM）和紫外-可见光（UV-vis）分析对其进行了表征。有趣的是，Gen的加入大大提高了Ag+的还原效率。采用致病性金黄色葡萄球菌（S。金黄色葡萄球菌）细菌产生的生物膜，因此，我们发现低浓度负载，Ag+的持久释放（28天），和抗菌效率的10倍的改善，实现了我们的新的AgNPs和基因嵌入的丝素蛋白涂层。在细菌和细胞共培养体系中，AgNPs/Gen包埋涂层对S.金黄色葡萄球菌，同时改善细胞粘附和生长。为了研究细胞相容性和成骨潜力，不同的涂层与MC 3 T3细胞培养; AgNPs/Gen包埋涂层显示出普遍可接受的生物相容性（细胞粘附，增殖和活力）和加速成骨细胞成熟（碱性磷酸酶的产生，基质分泌和钙化）。这种新型的生物功能涂层具有良好的抗菌、生物相容性和成骨活性，有望在骨科和牙科钛植入物中得到应用。
With the progressively increasing demand for orthopedic Ti implants, the balance between two primary complications restricting implant applications is needed to be solved: the lack of bone tissue integration and biomedical device-associated infections (BAI), where emergence of multiresistance bacteria make it worse. Notably, a combination of silver nanoparticles (AgNPs) and a kind of antibiotic can synergistically inhibit bacterial growth, where a low concentration of AgNPs has been confirmed to promote the proliferation and osteogenesis of osteoblasts. In this work, we built AgNPs/gentamicin (Gen)-embedded silk fibroin (SF)-based biomimetic coatings on orthopedic titanium by a facile dipping–drying circular process and with the assistance of polydopamine (PD). Ag+was reduced to AgNPs by SF under ultraviolet (UV) irradiation, and then they were detected by transmission electron microscope (TEM) images and UV–visible (UV–vis) analyses. Intriguingly, the addition of Gen highly improved the reduction efficiency of Ag+. The antibacterial efficiency of SF-based coatings was examined by challenging them with pathogenicStaphylococcus aureus(S. aureus) bacteria which produced biofilms, and consequently, we found that low concentration loading, durable release of Ag+(28 days), and 10-fold improvement of antibacterial efficiency were achieved for our novel AgNPs- and Gen-embeded silk fibroin coatings. In bacteria and a cells cocultured system, AgNPs/Gen-embedded coatings strongly inhibited adhesion and proliferation ofS. aureus, simultaneously improving cell adhesion and growth. To investigate cytocompatibility and osteogenic potential, different coatings were cultured with MC3T3 cells; AgNPs/Gen-embedded coatings showed generally acceptable biocompatibility (cell adhesion, proliferation, and viability) and accelerated osteoblast maturation (alkaline phosphatase production, matrix secretion, and calcification). Expectantly, this novel biofunctional coating will have promising applications in orthopedic and dental titanium implants thanks to its excellently antibacterial, biocompatible, and osteogenic activities.