POSTERIOR HYALOID DETACHMENT AND INTERNAL LIMITING MEMBRANE PEELING ASSISTED BY ANTHOCYANINS FROM ACAI FRUIT (EUTERPE OLERACEA) AND 10 OTHER NATURAL VITAL DYES Experimental Study in Cadaveric Eyes
POSTERIOR HYALOID DETACHMENT AND INTERNAL LIMITING MEMBRANE PEELING ASSISTED BY ANTHOCYANINS FROM ACAI FRUIT (EUTERPE OLERACEA) AND 10 OTHER NATURAL VITAL DYES Experimental Study in Cadaveric Eyes
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DOI：
10.1097/iae.0b013e3182618a6d
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发表时间：
2013-01-01
影响因子：
3.3
通讯作者：
Maia, Mauricio
中科院分区：
文献类型：
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作者：
Chen, Jane;Ferreira, Magno Antonio;Maia, Mauricio
目的：本研究的目的是确定是否天然染料促进后玻璃体脱离（后玻璃体脱离[PVD]）和视网膜内界膜（ILM）剥离在human eyes.Methods：开放天空玻璃体切除术后玻璃体和ILM去除在86人尸体眼。核心玻璃体切除术后，11种不同的染料注入玻璃体腔，以帮助玻璃体脱离和ILM去除。在PVD后使染料在黄斑上沉降5分钟，并通过机械抽吸除去。使用眼内镊进行ILM剥离，通过剥离组织的光学显微镜检查证实。猕猴桃（Euterpeoleracea）提取物和来自植物或动物来源的10种另外的染料进行了测试：石榴（Punica granatum），logwood（Haematoxylum campechianum），苜蓿叶绿素提取物（Medicago sativa）、胭脂虫红（Daclampius coccus），木槿属（Hibiscus rosa-sinensis）、靛蓝结果：染料促进PVD和ILM剥离。鸭梨（E. oleracea）提取物、苏木（H. campechianum）、胭脂虫红（D. coccus）和老褐腐霉M.着色剂）在所有情况下促进PVD;将染料辅助PVD与先前在比较模型中进行的曲安西龙辅助PVD进行比较。鸭梨（E. oleracea）提取物、胭脂虫红（D. coccus）和苜蓿叶绿素提取物（M. Sativa）显示出最好的ILM染色能力;将染料辅助的ILM去除与先前在比较模型中进行的由吲哚菁绿色染色引导的ILM剥离进行比较。结论：巴西莓果实（E。oleracea）和胭脂虫红（D.球菌）和苜蓿叶绿素提取物（M. sativa）对人尸体眼后玻璃体和ILM的染色能力最好，可能是玻璃体视网膜手术的有用工具。视网膜33：89-96，2013
Purpose: The purpose of this study was to determine whether natural dyes facilitate posterior hyaloid detachment (posterior vitreous detachment [PVD]) and retinal internal limiting membrane (ILM) peeling in human eyes.Methods: Open-sky vitrectomy with posterior hyaloid and ILM removal was performed in 86 human cadaveric eyes. After core vitrectomy, 11 different dyes were injected into the vitreous cavity to aid hyaloid detachment and ILM removal. The dyes were allowed to settle on the macula for 5 minutes after PVD and were removed by mechanical aspiration. Intraocular forceps were used for ILM peeling, which was confirmed by light microscopy of the peeled tissue. Acai fruit (Euterpe oleracea) extract and 10 additional dyes from plants or animal sources were tested: pomegranate (Punica granatum), logwood (Haematoxylum campechianum), chlorophyll extract from alfalfa (Medicago sativa), cochineal (Dactylopius coccus), hibiscus (Hibiscus rosa-sinensis), indigo (Indigofera tinctoria), paprika (Capiscum annuum), turmeric (Curcuma longa), old fustic (Maclura tinctoria), and grape (Vitis vinifera).Results: The dyes facilitated PVD and ILM peeling. Acai fruit (E. oleracea) extract, logwood (H. campechianum), cochineal (D. coccus), and old fustic (M. tinctoria) facilitated PVD in all cases; dye-assisted PVD was compared with triamcinolone-assisted PVD performed previously in a comparative model. Acai fruit (E. oleracea) extract, cochineal (D. coccus), and chlorophyll extract from alfalfa (M. sativa) showed the best capability for ILM staining; dye-assisted ILM removal was compared with the ILM peeling guided by indocyanine green staining performed previously in a comparative model. Light microscopy confirmed the ILM removal in all cases.Conclusion: Anthocyanin dye of the acai fruit (E. oleracea) and the dyes from cochineal (D. coccus) and chlorophyll extract from alfalfa (M. sativa) resulted in the best capability for posterior hyaloid and ILM staining in human cadaveric eyes and may be a useful tool for vitreoretinal surgery. RETINA 33:89-96, 2013