Chiral discrimination of the Japanese beetle sex pheromone and a behavioral antagonist by a pheromone-degrading enzyme
Chiral discrimination of the Japanese beetle sex pheromone and a behavioral antagonist by a pheromone-degrading enzyme
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DOI：
10.1073/pnas.0802610105
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发表时间：
2008-07-01
影响因子：
11.1
通讯作者：
Leal, Walter S.
中科院分区：
文献类型：
--
作者：
Ishida, Yuko;Leal, Walter S.
昆虫嗅觉系统的复杂性在日本甲虫接受性信息素的过程中得到了很好的证明。在这种昆虫，两个嗅觉受体神经元安置在前感器placodea是高度敏感的。一个神经元特异性地检测同种雌性产生的性信息素（R，Z）-5-(-)-（1-癸基）氧环戊烷-2- One [(R)-japonilure]。另一个神经元被调节到(S)-japonilure，这是一种性信息素，来自一个密切相关的物种，是日本甲虫的行为拮抗剂。这些化学信号被触角酯酶终止，酯酶打开内酯环形成生理上无活性的羟基酸。我们从日本甲虫的10万个触角中分离出了一种信息素降解酶PjapPDE。PjapPDE被证明仅在包含信息素检测感受器的天线组织中表达。杆状病毒表达产生重组PjapPDE，其翻译后修饰可能与天然酶相同。纯天然和重组PjapPDE的动力学研究显示出明显的底物偏好，性信息素和行为拮抗剂的体内半衰期分别约为30 ms和90 ms。
The sophistication of the insect olfactory system is elegantly demonstrated by the reception of sex pheromone by the Japanese beetle. In this insect, two olfactory receptor neurons housed in antermal sensilla placodea are highly sensitive. One neuron specifically detects the sex pheromone produced by conspecific females (R,Z)-5-(-)-(1-decenyl)oxacyclopentan-2-one [(R)-japonilure]. The other neuron is tuned to (S)-japonilure, a sex pheromone from a closely related species and a behavioral antagonist for the Japanese beetle. These chemical signals are enzymatically terminated by antennal esterases that open the lactone rings to form physiologically inactive hydroxyacids. We have isolated a pheromone-degrading enzyme, PjapPDE, from >100,000 antennae of the Japanese beetle. PjapPDE was demonstrated to be expressed only in the antenna[ tissues housing the pheromone-detecting sensilla placodea. Baculovirus expression generated recombinant PjapPDE with likely the same posttranslational modifications as the native enzyme. Kinetic studies with pure native and recombinant PjapPDE showed a clear substrate preference, with an estimated half-life in vivo for the sex pheromone and a behavioral antagonist of approximate to 30 and approximate to 90 ms, respectively.