Tissue-specific and ectopic expression of genes introduced into transgenic mice by retroviruses.
Tissue-specific and ectopic expression of genes introduced into transgenic mice by retroviruses.
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通过逆转录病毒导入转基因小鼠的基因的组织特异性和异位表达。
DOI：
10.1126/science.3024318
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Jaenisch,R
中科院分区：
文献类型：
--
作者：
Soriano,P;Cone,RD;Mulligan,RC;Jaenisch,R
用含有完整基因组人β珠蛋白基因(由自身启动子控制)和细菌新霉素磷酸转移酶基因(由正常或非增强病毒启动子控制)的重组逆转录病毒通过感染植入前胚胎获得转基因小鼠株系。在所有转基因菌株中都观察到β珠蛋白基因在造血组织中的表达。此外，有一株病毒株在同一组织中异位表达β珠蛋白，该组织也表达高水平的病毒启动子。与来自内部启动子的表达相反，来自长末端重复序列(LTR)的表达很可能依赖于整合位置。因此，逆转录病毒载体可用于转基因小鼠中外源基因的组织特异性表达，以及用于鉴定允许前病毒发育激活的基因座。
Recombinant retroviruses containing the complete genomic human β globin gene (under the control of its own promoter) and the bacterial neomycin phosphotransferase gene (under the control of the normal or enhancerless viral promoter) were used to derive transgenic mouse strains by infection of preimplantation embryos. Expression of the β globin gene in hematopoietic tissues was observed in all transgenic strains. In addition, one strain showed ectopic expression of β globin in the same tissues that also expressed high levels of RNA from the viral promoter. It is likely that expression from the long terminal repeat (LTR), in contrast to expression from the internal promoter, is dependent on the site of integration. Thus, retroviral vectors can be used for tissue-specific expression of foreign genes in transgenic mice, as well as for the identification of loci that allow developmental activation of a provirus.