The role of UCP 1 in production of reactive oxygen species by mitochondria isolated from brown adipose tissue
The role of UCP 1 in production of reactive oxygen species by mitochondria isolated from brown adipose tissue
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DOI：
10.1016/j.bbabio.2010.04.008
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发表时间：
2010-08-01
影响因子：
4.3
通讯作者：
Porter, Richard K.
中科院分区：
文献类型：
--
作者：
Dlaskova, Andrea;Clarke, Kieran J.;Porter, Richard K.
我们提供的证据表明，消融或抑制，解偶联蛋白1增加的速率，含活性氧的物种生产的线粒体从棕色脂肪组织，无论是什么电子传递链底物使用（琥珀酸，甘油-3-磷酸或丙酮酸/苹果酸）。与这些数据一致的是我们的观察结果，即（a）当解偶联蛋白1被消除或抑制时，线粒体膜电位最大，和（B）来自解偶联蛋白1敲除小鼠的线粒体中的耗氧速率显著低于来自野生型小鼠的那些，但与来自存在GDP的野生型小鼠的那些相当。总之，我们发现解偶联蛋白1可以影响棕色脂肪组织中分离的线粒体产生活性氧。(C)2010 Elsevier B. V.保留所有权利。
We provide evidence that ablation or inhibition of, uncoupling protein 1 increases the rate of reactive oxygen containing species production by mitochondria from brown adipose tissue, no matter what electron transport chain substrate is used (succinate, glycerol-3-phosphate or pyruvate/malate). Consistent with these data are our observations that (a) the mitochondrial membrane potential is maximal when uncoupling protein 1 is ablated or inhibited and (b) oxygen consumption rates in mitochondria from uncoupling protein 1 knock-out mice, are significantly lower than those from wild-type mice, but equivalent to those from wildtype mice in the presence of GDP. In summary, we show that uncoupling protein 1 can affect reactive oxygen containing species production by isolated mitochondria from brown adipose tissue. (C) 2010 Elsevier B.V. All rights reserved.