Regulatory role of pyruvate-sensing BtsSR in biofilm formation by Escherichia coli K-12
Regulatory role of pyruvate-sensing BtsSR in biofilm formation by Escherichia coli K-12
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DOI：
10.1093/femsle/fnz251
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发表时间：
2019-12-01
影响因子：
2.1
通讯作者：
Ishihama, Akira
中科院分区：
文献类型：
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作者：
Ogasawara, Hiroshi;Ishizuka, Toshiyuki;Ishihama, Akira
丙酮酸是多种代谢途径的关键调控因子，通过调控两个丙酮酸敏感双组分系统（TCS）BtsSR和PyrSR的活性来影响大肠杆菌基因组的转录。在此之前，我们确定了PyrSR与丙酮酸低亲和力的整套调控靶点。使用gSELEX筛选系统，我们发现BtsSR与丙酮酸高亲和力调节超过100个基因，包括多达13个转录因子基因，包括csgD基因编码的生物膜形成的主调节因子。CsgD调节超过20个靶基因，包括编码Curli菌毛的csg操纵子。此外，我们确定csgBAC是BtsR的调节靶点之一，从而表明卷曲形成涉及两种丙酮酸依赖性调节途径：CsgD的间接调节;和BtsR的直接调节。基于两个丙酮酸敏感的BtsR和PyrR的整套调控靶点的发现，我们进一步提出了一个创新的概念，即不同基因组的丙酮酸水平依赖性调控通过两个丙酮酸敏感的TCS系统，高亲和力的BtsSR和低亲和力的PyrSR丙酮酸。
Pyruvate, the key regulator in connection of a variety of metabolic pathways, influences transcription of the Escherichia coli genome through controlling the activity of two pyruvate-sensing two-component systems (TCSs), BtsSR and PyrSR. Previously, we identified the whole set of regulatory targets of PyrSR with low-affinity to pyruvate. Using gSELEX screening system, we found here that BtsSR with high-affinity to pyruvate regulates more than 100 genes including as many as 13 transcription factors genes including the csgD gene encoding the master regulator of biofilm formation. CsgD regulates more than 20 target genes including the csg operons encoding the Curli fimbriae. In addition, we identified the csgBAC as one of the regulatory targets of BtsR, thus indicating the involvement of two pyruvate-dependent regulatory pathways of the curli formation: indirect regulation by CsgD; and direct regulation by BtsR. Based on the findings of the whole set of regulatory targets by two pyruvate-sensing BtsR and PyrR, we further propose an innovative concept that the pyruvate level-dependent regulation of different gene sets takes place through two pyruvate-sensing TCS systems, high-affinity BtsSR and low-affinity PyrSR to pyruvate.