Environmental history impacts gene expression during diapause development in the alfalfa leafcutting bee, Megachile rotundata
Environmental history impacts gene expression during diapause development in the alfalfa leafcutting bee, Megachile rotundata
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DOI：
10.1242/jeb.173443
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发表时间：
2018-07
影响因子：
2.8
通讯作者：
G. Yocum;Anna K. Childers;J. Rinehart;A. Rajamohan;T. Pitts‐Singer;K. Greenlee;J. Bowsher
中科院分区：
文献类型：
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作者：
G. Yocum;Anna K. Childers;J. Rinehart;A. Rajamohan;T. Pitts‐Singer;K. Greenlee;J. Bowsher
摘要随着RNA测序等基因表达技术的引入，人们对昆虫滞育控制机制的认识大大提高。然而，很少有人注意到生态相关的领域条件可能会影响滞育发育过程中的基因表达，因为以前的研究都集中在实验室饲养和维护的昆虫。为了确定基因表达是否在实验室和田间条件下不同，苜蓿切叶蜂，切叶蜂圆切叶蜂，进入滞育早或晚在生长季节的包皮收集。这两组进一步细分为实验室和现场维护组，在初秋，导致滞育包皮的四个实验治疗：早期和晚期的领域，早期和晚期的实验室。在11月、1月、3月和5月对来自四个处理组的蜜蜂进行RNA-seq和差异表达分析。处理特异性差异表达基因的数量（97至1249）超过了所有四种处理（14至229）共有的差异调节基因的数量，表明暴露于实验室或田间条件对滞育发育期间的基因表达产生了重大影响。主成分分析和层次聚类分析产生了类似的分组的治疗，确认治疗形成不同的集群。我们的结果支持了这样的结论：滞育发育过程中的基因表达不是一个简单的有序序列，而是一种主要由个体昆虫的环境历史决定的高度可塑性反应。总结：圆切叶蜂滞育发育过程中的基因表达受环境历史的影响，并在个体越冬包皮之间存在高度差异。
ABSTRACT Our understanding of the mechanisms controlling insect diapause has increased dramatically with the introduction of global gene expression techniques, such as RNA sequencing (RNA-seq). However, little attention has been given to how ecologically relevant field conditions may affect gene expression during diapause development because previous studies have focused on laboratory-reared and -maintained insects. To determine whether gene expression differs between laboratory and field conditions, prepupae of the alfalfa leafcutting bee, Megachile rotundata, entering diapause early or late in the growing season were collected. These two groups were further subdivided in early autumn into laboratory- and field-maintained groups, resulting in four experimental treatments of diapausing prepupae: early and late field, and early and late laboratory. RNA-seq and differential expression analyses were performed on bees from the four treatment groups in November, January, March and May. The number of treatment-specific differentially expressed genes (97 to 1249) outnumbered the number of differentially regulated genes common to all four treatments (14 to 229), indicating that exposure to laboratory or field conditions had a major impact on gene expression during diapause development. Principle component analysis and hierarchical cluster analysis yielded similar grouping of treatments, confirming that the treatments form distinct clusters. Our results support the conclusion that gene expression during the course of diapause development is not a simple ordered sequence, but rather a highly plastic response determined primarily by the environmental history of the individual insect. Summary: Gene expression during diapause development in Megachile rotundata is impacted by environmental history and is highly variable between individual overwintering prepupae.