Syntheses of biotinylated alpha-galactosylceramides and their effects on the immune system and CD1 molecules.
Syntheses of biotinylated alpha-galactosylceramides and their effects on the immune system and CD1 molecules.
复制标题
生物素化 α-半乳糖神经酰胺的合成及其对免疫系统和 CD1 分子的影响。
DOI：
10.1021/jm990054n
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Koezuka,Y
中科院分区：
文献类型：
--
作者：
Sakai,T;Naidenko,OV;Iijima,H;Kronenberg,M;Koezuka,Y
代表性的α-半乳糖神经酰胺（α-GalCer）KRN 7000具有强的免疫刺激和抗肿瘤活性。最近的研究表明，KRN 7000脉冲的抗原呈递细胞（APC）可以激活自然杀伤T（NKT）细胞，一种新的T细胞谱系，APC上的CD 1d分子在NKT细胞的激活中起重要作用。然而，目前还不清楚α-GalCers是否真的与CD 1d分子结合。为了解决这个问题，我们合成了三种生物素化的α-GalCer和一种生物素化的β-GalCer，发现生物素化的α-GalCer能显著刺激小鼠脾细胞增殖，而生物素化的β-GalCer不能，并且所有生物素化的化合物都能与CD 1d分子结合。
A representative α-galactosylceramide (α-GalCer), KRN7000, has strong immunostimulatory and antitumor activity. Recent studies demonstrated that KRN7000-pulsed antigen-presenting cells (APC) can activate natural killer T (NKT) cells, a novel T-cell lineage, and CD1d molecules on APC play an important role in the activation of NKT cells. However, it remains unclear whether α-GalCers actually bind to CD1d molecules. To address this question, we synthesized three kinds of biotinylated α-GalCer and a biotinylated β-GalCer and found that the biotinylated α-GalCers significantly stimulate the proliferation of murine spleen cells, but the biotinylated β-GalCer does not and that all biotinylated compounds bind to CD1d molecules.