Photoreceptor allografts in a feline model of retinal degeneration
Photoreceptor allografts in a feline model of retinal degeneration
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DOI：
10.1007/s004170050169
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发表时间：
1998-11-01
影响因子：
2.7
通讯作者：
Narfstrom, K
中科院分区：
文献类型：
--
作者：
Ivert, L;Gouras, P;Narfstrom, K
背景资料：光感受器移植提供了一种潜在的手段来恢复退化视网膜的功能和/或通过营养影响拯救退化的宿主光感受器。我们已经在阿比西尼亚猫遗传性光感受器变性模型中检查了光感受器同种异体移植物，以确定这种移植物对宿主视网膜的活力和影响。将含有未分化光感受器的3- 5日龄正常小猫视网膜的小块，在视网膜变性的早期阶段，使用标准玻璃体显微镜注射到成年阿比西尼亚猫的视网膜下腔中。视网膜手术技术，术后不同时间行眼底镜检查和眼底照相，光镜和电镜检查。结果：同种异体移植物术后至少存活6个月。光感受器发育外节，总是在视网膜中。移植物逐渐与宿主视网膜整合，但使宿主光感受器层与视网膜色素上皮（RPE）分离，这倾向于减少移植物中宿主光感受器的数量。邻近未脱离视网膜的光感受器变性没有减缓。未检测到炎症或排斥反应。结论：未分化的新生感光细胞同种异体移植物在阿比西尼亚突变猫视网膜的视网膜下腔中存活并发育成外节。同种异体移植物逐渐与宿主神经视网膜整合而不诱导排斥反应。在移植物附近，宿主光感受器的损失增加，这被认为是由于它们从RPE层脱离。没有证据表明在这种突变视网膜的其他地方有任何宿主光感受器的拯救。
Background: Photoreceptor transplants provide a potential means to restore function in a degenerate retina and/or rescue degenerating host photoreceptors by trophic influences, We have examined photoreceptor allografts in the Abyssinian cat model of hereditary photoreceptor degeneration to determine the viability and influence of such transplants on the host retina.Methods: Small pieces of 3- to 5-day-old normal kitten retina containing undifferentiated photoreceptors were injected into the subretinal space of adult Abyssinian cats at an early stage of retinal degeneration using standard vitreo-retinal surgical techniques, The retinas were examined by ophthalmoscopy and fundus photography, then by light and electron microscopy at different times after surgery. Results: Such allografts survive for at least 6 months after surgery. The photoreceptors develop outer segments, invariably in rosettes, The transplants gradually integrate with the host retina but detach the host photoreceptor layer from the retinal pigment epithelium (RPE), which tends to reduce the number of host photoreceptors over the transplant. There is no slowing of the photoreceptor degeneration in neighboring non-detached retina. Inflammation or rejection was not detected. Conclusion: Undifferentiated, neonatal photoreceptor allografts survive and develop outer segments in the subretinal space of the Abyssinian mutant feline retina. The allografts gradually integrate with the host neural retina without inducing rejection. In the vicinity of the transplant there is increased loss of host photoreceptors, considered to be due to their detachment from the RPE layer. There is no evidence of any rescue of host photoreceptors elsewhere in this mutant retina.