Retrograde traffic out of the yeast vacuole to the TGN occurs via the prevacuolar/endosomal compartment.
Retrograde traffic out of the yeast vacuole to the TGN occurs via the prevacuolar/endosomal compartment.
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酵母液泡从TGN的逆行流量通过prevacuall/ensosomal室发生。
DOI：
10.1083/jcb.142.3.651
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发表时间：
1998-08-10
影响因子：
7.8
通讯作者：
Stevens, TH
中科院分区：
文献类型：
--
作者：
Bryant, NJ;Piper, RC;Weisman, LS;Stevens, TH
高尔基体 (TGN) 的最后一个隔室和酿酒酵母的液泡之间发生大量的运输步骤。迄今为止，已确定从 TGN 到液泡的两条细胞内途径。羧肽酶 Y (CPY) 穿过液泡前/内体区室 (PVC)，随后到达液泡，而碱性磷酸酶 (ALP) 则绕过该区室到达相同的细胞器。尽管流量持续不断，但驻留在 TGN 的蛋白质通过含有芳香族氨基酸的分选基序不断从远端 PVC 中检索来实现其定位。在这项研究中，我们报道了一种基于 ALP 并含有这种修复基序的杂合蛋白不是通过 CPY 分选途径到达 PVC，而是通过信号依赖性逆行转运从液泡（一种先前被认为是末端室的细胞器）中到达 PVC。此外，我们发现 VAC7 中的突变（之前被确定为液泡遗传所需的基因）阻止了这一运输步骤。最后我们表明，Vti1p（一种将 CPY 和 ALP 递送至液泡所需的 v-SNARE）使用逆行运输出液泡作为其正常细胞行程的一部分。
A large number of trafficking steps occur between the last compartment of the Golgi apparatus (TGN) and the vacuole of the yeast Saccharomyces cerevisiae. To date, two intracellular routes from the TGN to the vacuole have been identified. Carboxypeptidase Y (CPY) travels through a prevacuolar/endosomal compartment (PVC), and subsequently on to the vacuole, while alkaline phosphatase (ALP) bypasses this compartment to reach the same organelle. Proteins resident to the TGN achieve their localization despite a continuous flux of traffic by continually being retrieved from the distal PVC by virtue of an aromatic amino acid–containing sorting motif. In this study we report that a hybrid protein based on ALP and containing this retrieval motif reaches the PVC not by following the CPY sorting pathway, but instead by signal-dependent retrograde transport from the vacuole, an organelle previously thought of as a terminal compartment. In addition, we show that a mutation in VAC7, a gene previously identified as being required for vacuolar inheritance, blocks this trafficking step. Finally we show that Vti1p, a v-SNARE required for the delivery of both CPY and ALP to the vacuole, uses retrograde transport out of the vacuole as part of its normal cellular itinerary.
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多特异性语法同源物，VAM3P，对于自噬和生物合成蛋白传输到液泡必不可少。
影响因子：
7.8
作者：
Darsow, T;Rieder, SE;Emr, SD
通讯作者：
Emr, SD
影响因子：
7.8
作者：
FRANZUSOFF, A;REDDING, K;SCHEKMAN, R
通讯作者：
SCHEKMAN, R
影响因子：
11.4
作者：
Holthuis, JCM;Nichols, BJ;Pelham, HRB
通讯作者：
Pelham, HRB
影响因子：
11.4
作者：
Cowles, CR;Snyder, WB;Emr, SD
通讯作者：
Emr, SD
影响因子：
5.3
作者：
Bonangelino, CJ;Catlett, NL;Weisman, LS
通讯作者：
Weisman, LS