Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing.
Single-Cell RNA Sequencing Reveals B Cells Are Important Regulators in Fracture Healing.
复制标题
单细胞RNA测序揭示B细胞是骨折愈合的重要调节因子。
DOI：
10.3389/fendo.2021.666140
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发表时间：
2021
影响因子：
5.2
通讯作者：
Ji F
中科院分区：
文献类型：
--
作者：
Zhang H;Wang R;Wang G;Zhang B;Wang C;Li D;Ding C;Wei Q;Fan Z;Tang H;Ji F
骨髓微环境主要由在骨折愈合中起重要作用的免疫细胞和基质细胞组成。虽然免疫细胞已经在小鼠骨髓中被鉴定，但在骨折愈合过程中它们的数量和类型的变化仍然不清楚。在这项研究中，单细胞RNA测序用于鉴定骨折组织中的免疫细胞，包括中性粒细胞、单核细胞、T细胞、B细胞和浆细胞。骨折愈合早期B细胞数量明显减少。此外，小鼠骨折模型中的B细胞在骨骺期显著减少，然后在骨折愈合的骨骺转化期逐渐恢复正常。B细胞类型与骨形成和骨吸收活动相反。值得注意的是，B细胞来源的外泌体抑制骨折愈合中的骨稳态。在人类中，骨骺期B细胞数量的减少刺激骨折愈合。然后，在骨痂重建阶段，随着成骨细胞数量的增加，B细胞的数量逐渐恢复，这减少了额外的骨再生。因此，B细胞是骨折愈合的关键调节因子，并通过产生多种成骨细胞抑制剂来抑制过度的骨再生。
The bone marrow microenvironment is composed primarily of immune and stromal cells that play important roles in fracture healing. Although immune cells have been identified in mouse bone marrow, variations in their numbers and type during the fracture healing process remain poorly defined. In this study, single-cell RNA sequencing was used to identify immune cells in fracture tissues, including neutrophils, monocytes, T cells, B cells, and plasma cells. The number of B cells decreased significantly in the early stage of fracture healing. Furthermore, B cells in mice fracture models decreased significantly during the epiphyseal phase and then gradually returned to normal during the epiphyseal transformation phase of fracture healing. The B-cell pattern was opposite to that of bone formation and resorption activities. Notably, B-cell–derived exosomes inhibited bone homeostasis in fracture healing. In humans, a decrease in the number of B cells during the epiphyseal phase stimulated fracture healing. Then, as the numbers of osteoblasts increased during the callus reconstruction stage, the number of B cells gradually recovered, which reduced additional bone regeneration. Thus, B cells are key regulators of fracture healing and inhibit excessive bone regeneration by producing multiple osteoblast inhibitors.
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影响因子：
64.5
作者：
Baryawno, Ninib;Przybylski, Dariusz;Scadden, David T.
通讯作者：
Scadden, David T.
DOI：
10.1126/science.aad0501
发表时间：
2016-04-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Tirosh I;Izar B;Prakadan SM;Wadsworth MH 2nd;Treacy D;Trombetta JJ;Rotem A;Rodman C;Lian C;Murphy G;Fallahi-Sichani M;Dutton-Regester K;Lin JR;Cohen O;Shah P;Lu D;Genshaft AS;Hughes TK;Ziegler CG;Kazer SW;Gaillard A;Kolb KE;Villani AC;Johannessen CM;Andreev AY;Van Allen EM;Bertagnolli M;Sorger PK;Sullivan RJ;Flaherty KT;Frederick DT;Jané-Valbuena J;Yoon CH;Rozenblatt-Rosen O;Shalek AK;Regev A;Garraway LA
通讯作者：
Garraway LA
影响因子：
4.8
作者：
Onal, Melda;Xiong, Jinhu;O'Brien, Charles A.
通讯作者：
O'Brien, Charles A.
影响因子：
64.5
作者：
Gaublomme JT;Yosef N;Lee Y;Gertner RS;Yang LV;Wu C;Pandolfi PP;Mak T;Satija R;Shalek AK;Kuchroo VK;Park H;Regev A
通讯作者：
Regev A
影响因子：
64.8
作者：
Vento-Tormo R;Efremova M;Botting RA;Turco MY;Vento-Tormo M;Meyer KB;Park JE;Stephenson E;Polański K;Goncalves A;Gardner L;Holmqvist S;Henriksson J;Zou A;Sharkey AM;Millar B;Innes B;Wood L;Wilbrey-Clark A;Payne RP;Ivarsson MA;Lisgo S;Filby A;Rowitch DH;Bulmer JN;Wright GJ;Stubbington MJT;Haniffa M;Moffett A;Teichmann SA
通讯作者：
Teichmann SA