Inhibitory effects of a novel antimicrobial peptide from kefir against Escherichia coli
Inhibitory effects of a novel antimicrobial peptide from kefir against Escherichia coli
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DOI：
10.1016/j.foodcont.2016.01.023
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发表时间：
2016-07-01
期刊：
影响因子：
6
通讯作者：
Xiao, Hang
中科院分区：
文献类型：
--
作者：
Miao, Jianyin;Liu, Guo;Xiao, Hang
抗菌肽F1是藏灵菇中的一种新型抗菌肽，对多种细菌和真菌具有较强的抗菌活性。对F1肽的氨基酸序列进行了鉴定，并研究了F1肽对大肠杆菌的抗菌机理。结果表明，抗菌肽F1由18个氨基酸组成（Thr-DAP-Asn-Thr-PEA-His-Pro-Asn-Thr-His-Leu-Ile- PEA-CySHVal-Asn-PEA-Tau），具有增强大肠杆菌外膜和内膜通透性的作用。在此过程中检测到胞浆β-半乳糖苷酶和钾离子的渗漏。E.通过激光共聚焦显微镜和透射电子显微镜观察抗菌肽F1对大肠杆菌的作用，结果表明抗菌肽F1可以渗透并积聚到细胞内，破坏细胞膜功能。此外，我们阐明了DNA的发现，抗菌肽F1具有多个目标，在杀死E。杆菌(C)2016爱思唯尔有限公司版权所有。
Antimicrobial peptide F1, a novel antimicrobial peptide from Tibetan kefir, have shown strong antimicrobial activity against several bacteria and fungi. We identified the amino acid sequence and studied the antimicrobial mechanism of peptide F1 against Escherichia coli. Our results showed that antimicrobial peptide F1 contained 18 amino acids (Thr-DAP-Asn-Thr-PEA-His-Pro-Asn-Thr-His-Leu-Ile- PEA-CySHVal-Asn-PEA-Tau), which increased the outer and inner membrane permeability of E. coli, and the leakage of the cytoplasmic beta-galactosidase and potassium ions was detected in the process. Morphologies of E. coli were observed by confocal laser scanning microscopy and transmission electron microscopy, which visually showed that antimicrobial peptide F1 could penetrate and accumulate into cell causing disruption of cell membrane functions. Furthermore, we elucidated the DNA findings in that antimicrobial peptide F1 has multiple targets in the killing of E. coli. (C) 2016 Elsevier Ltd. All rights reserved.