RUNX3 copy number predicts the development of UC-associated colorectal cancer.
RUNX3 copy number predicts the development of UC-associated colorectal cancer.
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DOI：
10.3892/ijo_00000839
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发表时间：
2010-11
影响因子：
5.2
通讯作者：
Toshiaki Watanabe;T. Kobunai;H. Ikeuchi;Yoko Yamamoto;K. Matsuda;S. Ishihara;K. Nozawa;H. Iinuma;T. Kanazawa;Toshiaki Tanaka;T. Yokoyama;T. Konishi;K. Eshima;Y. Ajioka;T. Hibi;Mamoru Watanabe;T. Muto;H. Nagawa
中科院分区：
文献类型：
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作者：
Toshiaki Watanabe;T. Kobunai;H. Ikeuchi;Yoko Yamamoto;K. Matsuda;S. Ishihara;K. Nozawa;H. Iinuma;T. Kanazawa;Toshiaki Tanaka;T. Yokoyama;T. Konishi;K. Eshima;Y. Ajioka;T. Hibi;Mamoru Watanabe;T. Muto;H. Nagawa
RUNX 3是一种肿瘤抑制基因，在各种癌症的发展中起着重要作用。本研究旨在比较溃疡性结肠炎（UC）伴和不伴UC相关结直肠癌（UC-Ca）患者的非肿瘤性直肠粘膜中RUNX 3 mRNA表达水平和DNA拷贝数。我们进一步旨在建立基于RUNX 3 DNA拷贝数的UC-Ca发展的预测模型。通过RT-PCR定量RUNX 3 mRNA表达水平。还测定了RUNX 3的超甲基化和DNA拷贝数。检查了35例UC患者，其中17例有UC-Ca（UC-Ca组），18例无UC-NonCa组）。UC-Ca组的mRUNX 3表达水平和DNA拷贝数显著低于UC-NonCa组（分别为p=0.04，p=0.0016）。RUNX 3表达水平与DNA拷贝数相关。基于DNA拷贝数的UC-Ca和UC-NonCa组的分类准确率为82.9%。在UC-Ca组中，非肿瘤性直肠粘膜中的RUNX 3表达水平显著降低，这表明这可归因于RUNX 3 DNA拷贝数的减少。本预测模型可用于筛选高危UC-Ca患者，并提高监测结肠镜检查的效果。本研究提示RUNX 3可能在UC-Ca的发生发展中起重要作用。
RUNX3 is a tumour suppressor gene that plays an important role in the development of various cancers. The present study aimed to compare RUNX3 mRNA expression levels and DNA copy numbers in the non-neoplastic rectal mucosa between ulcerative colitis (UC) patients with and without UC-associated colorectal cancer (UC-Ca). We further aimed to build a predictive model of the development of UC-Ca based on the RUNX3 DNA copy number. RUNX3 mRNA expression levels were quantified by RT-PCR. The hypermethylation and DNA copy number of RUNX3 were also determined. Thirty-five UC patients were examined, 17 of whom had UC-Ca (UC-Ca group) and 18 who did not (UC-NonCa group). The UC-Ca group had significantly lower mRUNX3 expression levels and smaller DNA copy numbers than the UC-NonCa group (p=0.04, p=0.0016, respectively). RUNX3 expression levels correlated with DNA copy numbers. Classification of the UC-Ca and UC-NonCa group based on DNA copy number gave an accuracy of 82.9%. RUNX3 expression levels in the non-neoplastic rectal mucosa was significantly decreased in the UC-Ca group and it is suggested that this was attributable to the decrease in RUNX3 DNA copy number. The present predictive model may be useful in the selection of high risk UC-Ca patients and to improve the efficacy of surveillance colonoscopy. The present study suggests that RUNX3 might play an important role in the development of UC-Ca.