The Drosophila cohesin subunit Rad21 is a trithorax group (trxG) protein
The Drosophila cohesin subunit Rad21 is a trithorax group (trxG) protein
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DOI：
10.1073/pnas.0801698105
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发表时间：
2008-08-26
影响因子：
11.1
通讯作者：
Honda, Barry M.
中科院分区：
文献类型：
--
作者：
Hallson, Graham;Syrzycka, Monika;Honda, Barry M.
黏连蛋白复合物是调节细胞分裂的关键因素。粘着蛋白SMC 1、SMC 3、Rad 21和基质蛋白（SA），沿着相关蛋白Nipped-B、Pds 5和Ecol，在分裂后期分离成子细胞之前维持姐妹染色单体的粘着。最近的染色质免疫沉淀（ChIP）的数据显示，广泛的重叠的Nipped-B和粘附素成分与RNA聚合酶II结合在果蝇的活性基因。这些和其他数据强烈表明，在转录的凝聚力的作用，但是，有没有明确的证据表明，任何特定的机制，凝聚素和相关蛋白质调节转录。在这里，我们报告之间的联系，凝聚素组件和trithorax组（trxG）的功能，从而牵连这些蛋白质的转录激活和/或延伸。我们表明，果蝇Rad 21蛋白编码的trxG基因家族的成员，也参与调节刺猬（hh）基因的trxG（vtd）。此外，相关蛋白Nipped-B中的突变显示出类似的trxG活性，即，与vtd一样，它们作为Pc和hh（Mrt）的显性抑制因子而不损害细胞分裂。我们的研究结果提供了一个框架，以进一步研究如何cohesin和相关组件可能调节转录。
The cohesin complex is a key player in regulating cell division. Cohesin proteins SMC1, SMC3, Rad21, and stromalin (SA), along with associated proteins Nipped-B, Pds5, and Ecol, maintain sister chromatid cohesion before segregation to daughter cells during anaphase. Recent chromatin immunoprecipitation (ChIP) data reveal extensive overlap of Nipped-B and cohesin components with RNA polymerase II binding at active genes in Drosophila. These and other data strongly suggest a role for cohesion in transcription; however, there is no clear evidence for any specific mechanisms by which cohesin and associated proteins regulate transcription. We report here a link between cohesin components and trithorax group (trxG) function, thus implicating these proteins in transcription activation and/or elongation. We show that the Drosophila Rad21 protein is encoded by verthandi (vtd), a member of the trxG gene family that is also involved in regulating the hedgehog (hh) gene. In addition, mutations in the associated protein Nipped-B show similar trxG activity i.e., like vtd, they act as dominant suppressors of Pc and hh(Mrt) without impairing cell division. Our results provide a framework to further investigate how cohesin and associated components might regulate transcription.