U2 RNA FROM YEAST IS UNEXPECTEDLY LARGE AND CONTAINS HOMOLOGY TO VERTEBRATE U4, U5, AND U6 SMALL NUCLEAR RNAS
U2 RNA FROM YEAST IS UNEXPECTEDLY LARGE AND CONTAINS HOMOLOGY TO VERTEBRATE U4, U5, AND U6 SMALL NUCLEAR RNAS
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DOI：
10.1016/0092-8674(86)90365-x
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发表时间：
1986-10-10
期刊：
影响因子：
64.5
通讯作者：
ARES, M
中科院分区：
文献类型：
--
作者：
ARES, M
我已经确定了酿酒酵母中编码脊椎动物 U2 snRNA 酵母同源物的基因的结构。令人惊讶的是，该 RNA 长 1175 个核苷酸，比其他生物体（包括粟酒裂殖酵母）的 U2 RNA 大六倍。大RNA的近100个核苷酸与后生动物U2具有序列同源性和潜在的二级结构。大RNA还包含与脊椎动物U4、U5和U6 snRNA的同源性，这意味着包含大RNA的RNP具有“多-snRNP”结构。编码大RNA的基因LSR1对于生长至关重要，这表明可以使用遗传方法来解剖酵母剪接体。剪接体 RNA 的不同组织可能是酵母和后生动物之间剪接差异的基础。
I have determined the structure of the gene from Saccharomyces cerevisiae coding for the yeast homolog of vertebrate U2 snRNA. Surprisingly, the RNA is 1175 nucleotides long, six times larger than U2 RNAs from other organisms, including Schizosaccharomyces pombe. Nearly 100 nucleotides of the large RNA share sequence homology and potential secondary structure with metazoan U2. The large RNA also contains homology to vertebrate U4, U5, and U6 snRNAs, implying a "poly-snRNP" structure for the RNP containing the large RNA. The gene LSR1, encoding the large RNA is essential for growth, suggesting that the yeast spliceosome can be dissected using genetic approaches. The different organization of spliceosomal RNA may underlie differences in splicing between yeast and metazoans.