The colonic metabolites dihydrocaffeic acid and dihydroferulic acid are more effective inhibitors of in vitro platelet activation than their phenolic precursors
The colonic metabolites dihydrocaffeic acid and dihydroferulic acid are more effective inhibitors of in vitro platelet activation than their phenolic precursors
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DOI：
10.1039/c6fo01404f
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发表时间：
2017-03-01
期刊：
影响因子：
6.1
通讯作者：
de Roos, Baukje
中科院分区：
文献类型：
--
作者：
Baeza, Gema;Bachmair, Eva-Maria;de Roos, Baukje
心血管疾病（CVD）是全球发病率和死亡率的主要原因。食用富含多酚的健康饮食与CVD的发展呈负相关。本研究评价了绿色咖啡豆提取物（GCBE）和巴拉圭茶酚提取物（YMPE）、主要酚类和甲基黄嘌呤成分（5-咖啡酰奎宁酸、3，5-二咖啡酰奎宁酸、咖啡因和可可碱）及其主要代谢产物（咖啡酸、阿魏酸、二氢咖啡酸（DHCA）和二氢阿魏酸（DHFA））对体外血小板活化的影响。在与不同剂量（0.01-100 μ g/mL（-1）或μ M）的每种化合物孵育后，使用全血流式细胞术测定腺苷5 '-二磷酸诱导的P-选择素表达和纤维蛋白原结合。血小板P-选择素的表达显着降低YMPE和所有酚和甲基黄嘌呤成分在生理浓度，与对照组相比，而血小板上的纤维蛋白原结合显着增加。结肠代谢产物（DHCA和DHFA）对P-选择素表达的抑制作用比其酚类前体更强，表明酚类化合物代谢调节血小板活化的功效增加。
Cardiovascular disease (CVD) is the major cause of morbidity and mortality worldwide. The consumption of a healthy diet rich in polyphenols has been inversely associated with the development of CVD. This study evaluated the effects of green coffee bean extract (GCBE) and yerba mate phenolic extract (YMPE), the main phenolic and methylxanthine constituents (5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, caffeine, and theobromine), and their main metabolites (caffeic acid, ferulic acid, dihydrocaffeic acid (DHCA) and dihydroferulic acid (DHFA)) on platelet activation in vitro. Upon incubation with different doses (0.01-100 mu g mL(-1) or mu M) of each compound, adenosine 5'-diphosphate-induced P-selectin expression and fibrinogen binding were determined using whole blood flow cytometry. Platelet P-selectin expression was significantly decreased by YMPE and all phenolic and methylxanthine constituents at physiological concentrations, compared with control, whereas fibrinogen binding on platelets was significantly increased. The colonic metabolites (DHCA and DHFA) had stronger inhibitory effects on P-selectin expression than their phenolic precursors, suggesting an increase in the efficacy to modulate platelet activation with the metabolism of the phenolic compounds.