Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis
Expression of N-acetylglucosaminyltransferase V in gastric cancer correlates with metastasis and prognosis
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DOI：
10.3892/ijo.2014.2248
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发表时间：
2014-03-01
影响因子：
5.2
通讯作者：
Wang, Zhirong
中科院分区：
文献类型：
--
作者：
Huang, Binbin;Wu, Qiong;Wang, Zhirong
N-乙酰葡糖胺转移酶V（GnT-V）是催化细胞蛋白的天冬酰胺（N）连接的寡糖（N-聚糖）上的N-乙酰葡糖胺的β 1-6分支的酶，并且其功能障碍是各种癌的共同特征。然而，GnT-V的作用仍然存在争议。因此，GnT-V表达的临床意义在每种癌症类型中可能不同。GnT-V状态在胃癌患者中的意义尚未得到广泛研究。在本研究中，我们研究了GnT-V在胃癌标本中的表达在蛋白和mRNA水平。我们比较了GnT-V的表达与临床和病理变量。生成Kaplan-Meier生存曲线以显示原因特异性生存。此外，小干扰RNA被设计用于下调SGC 7901和BGC 823细胞中GnT-V mRNA的表达。我们通过细胞增殖和侵袭性分析来表征GnT-V的功能意义。胃癌组织中GnT-V的表达与肿瘤的分级和分期有关。GnT-V表达阳性胃癌患者的总生存期明显短于GnT-V表达阴性者。小分子干扰RNA下调GnT-V表达后，胃癌细胞株SGC 7901和胃癌细胞株BGC 823的增殖能力和侵袭力均下降，并伴有形态学改变。这支持GnT-V与胃癌的转移和预后相关。这些结果有助于新的洞察潜在的翻译临床应用在胃癌GnT-V调节的分子机制。
N-acetylglucosaminyltransferase V (GnT-V) is an enzyme that catalyzes beta 1-6 branching of N-acetylglucosamine on asparagine (N)-linked oligosaccharides (N-glycan) of cell proteins and the dysfunction of which is a common feature of various carcinomas. Nevertheless, the role of GnT-V remains controversial. Therefore, the clinical implication of GnT-V expression may differ in each cancer type. The implication of GnT-V status in patients with gastric cancer has not been studied extensively. In the present study, we examined GnT-V expression in gastric cancer specimen both at protein and mRNA levels. We compared GnT-V expression with clinical and pathologic variables. Kaplan-Meier survival curves were generated to show the cause-specific survival. Furthermore, the small interfering RNA was devised to downregulate the GnT-V mRNA expression in SGC7901 and BGC 823 cells. We characterized the function implication of GnT-V by cell proliferation and invasiveness analysis. Analysis in gastric cancer specimen revealed that GnT-V expression correlated with tumor grade and stage. The overall survival time of positive GnT-V expression in gastric cancer was significantly shorter than that of negative GnT-V expression. Moreover, the down-regulation of GnT-V expression by small interfering RNA resulted in a decrease of cell proliferation and invasiveness in SGC7901 and BGC 823 cells accompanied by morphological change. This supports that GnT-V correlates with metastasis and prognosis in gastric cancer. These results contribute to new insight into the underlying molecular mechanisms of GnT-V regulation in gastric cancer with potential translational clinical applications.