Technique for estimating fetal mouse thoracic volumes through image analysis of histological sections.
Technique for estimating fetal mouse thoracic volumes through image analysis of histological sections.
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通过组织切片图像分析估计胎儿小鼠胸廓体积的技术。
DOI：
10.1002/ar.1092250213
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Seegmiller,RE
中科院分区：
文献类型：
--
作者：
Hepworth,WB;Carter,MW;Seegmiller,RE
在之前的一项研究中，我们使用石蜡铸件估计了胎儿小鼠的胸腔体积。虽然该程序提供了有用的信息，但它不允许对胸部内脏进行组织学检查。在本研究中，通过连续组织学切片确定第 14-18 天胎儿小鼠的胸腔体积。从切片中追踪胸腔，并通过计算机图像分析确定每次追踪的面积。将这些面积相加，然后乘以每个部分的厚度即可得出胸腔体积。因此，该程序允许对胸内脏进行体积测定和组织学检查。此外，还比较了两种旨在提高此类体积估计实用性的随机抽样方法的可靠性。最适合本研究的方法是随机分层抽样方法，因为它以最小的标准偏差再现估计值。
In a previous study we estimated fetal mouse thoracic volume by use of paraffin casts. While this procedure provided useful information, it did not allow histologic examination of thoracic viscera. In the present study the thoracic volumes of day 14–18 fetal mice were determined through serial histological sections. The thoracic cavity was traced from the sections and the area of each tracing was determined by computer image analysis. These areas were summed and then multiplied by the thickness of each section to derive the thoracic volume. This procedure thus permitted both volumetric determinations and histological inspection of the thoracic viscera. In addition, two randomized sampling methods designed to increase the utility of such volumetric estimates were compared for reliability. The method best suited for this study was a random stratified sampling method because it reproduced estimates with minimal standard deviation.