Immunomodulating activities of polysaccharides isolated from Panax ginseng
Immunomodulating activities of polysaccharides isolated from Panax ginseng
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DOI：
10.1089/109662004322984626
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发表时间：
2004-03-01
影响因子：
2.4
通讯作者：
Hwang, JK
中科院分区：
文献类型：
--
作者：
Lim, TS;Na, K;Hwang, JK
人参迈耶传统上用于预防和治疗各种慢性疾病和感染。人参是人参提取过程后残留的纤维状和不溶性副产品。本研究采用挤压膨化法对人参果渣进行了结构分解，并分离得到了水溶性人参多糖（GMP）。在小鼠腹腔巨噬细胞中检查GMP的免疫调节作用。GMP能显著提高腹腔巨噬细胞的吞噬指数和溶酶体磷酸酶活性（P <0.05）。经GMP处理的腹腔巨噬细胞产生的H_2O_2和亚硝酸盐也显著高于未经GMP处理的对照组（P <0.05）。此外，GMP（100 μ g/mL）显著增加腹腔巨噬细胞的细胞活力（P <0.05）。这些结果表明，GMP是一种有效的非特异性免疫调节剂，其免疫刺激作用可能是由于其能够刺激活性氧中间体的产生。
Panax ginseng C.A. Meyer has been traditionally used for the prevention and treatment of various chronic diseases and infections. Ginseng mare is a fibrous and insoluble by-product remaining after the extraction process of ginseng. In this research an extrusion process was employed to disintegrate the insoluble ginseng marc structure, and water-soluble ginseng mare polysaccharide (GMP) was isolated. GMP was examined for immunomodulatory effects in murine peritoneal macrophages. GMP significantly increased the lysosomal phosphatase activity and the phagocytic index of peritoneal macrophages (P < .05). The peritoneal macrophages treated with GMP also produced significantly more H2O2 and nitrite than the control without GMP treatment (P < .05). In addition, GMP (100 mug/mL) significantly increased the cell viability of peritoneal macrophages (P < .05). These results suggest that GMP is an effective nonspecific immunomodulatory agent, and its immunostimulating effects may be due to its ability to stimulate the production of reactive oxygen intermediates.