Effects of chlorhexidine on bonding durability of different adhesive systems using a novel thermocycling method

Effects of chlorhexidine on bonding durability of different adhesive systems using a novel thermocycling method
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使用新型热循环方法,氯己定对不同粘合剂体系粘合耐久性的影响

DOI:
10.1111/adj.12038
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发表时间:
2013-06-01
影响因子:
2.1
通讯作者:
Pei, D.
Pei, D.
中科院分区:
医学4区
文献类型:
--
作者:
Deng, D.;Huang, X.;Pei, D.

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本研究的目的是使用聚合酶链反应(PCR)热循环方法评价洗必泰对蚀刻-冲洗和自蚀刻粘合剂系统的粘合耐久性的影响。方法将二十颗新鲜拔除的完整人类第三磨牙研磨并使用蚀刻冲洗粘合剂(Single-Bond)或自蚀刻粘合剂(G-Bond)粘合。标本要么保持不动,要么置于装有三种热循环介质(水、氯己定或硅油)的PCR管中。使用PCR程序在5 ℃和55 ℃的温度下进行热循环(5000个循环)。微拉伸粘结强度(TBS)进行了评价和界面纳米泄漏进行了评估,通过扫描电子显微镜之前和之后的热循环。结果热循环后,不同培养液组间差异有显著性。对于Single-Bond,氯己定和硅油基团均能保护TBS(p
Background The purpose of this study was to evaluate the influence of chlorhexidine on the bonding durability of etch-and-rinse and self-etch adhesive systems using the polymerase chain reaction (PCR) thermocycling method. Methods Twenty freshly extracted intact human third molars were ground and bonded with either an etch-and-rinse adhesive (Single-Bond) or a self-etch adhesive (G-Bond). Specimens were either left untouched or placed in PCR tubes filled with three thermocycling mediums: water, chlorhexidine or silicone oil. Thermocycling (5000 cycles) was done using the PCR programme at temperatures of 5 degrees C and 55 degrees C. The microtensile bond strength (TBS) was evaluated and interfacial nanoleakage was assessed by scanning electron microscopy before and after thermocycling. Results Significant differences were detected among groups kept in different media after thermocycling. For Single-Bond, both the chlorhexidine and silicone oil groups could preserve the TBS (p