Roles of bacterial attachment and spontaneous partitioning in the biodegradation of naphthalene initially present in nonaqueous-phase liquids
Roles of bacterial attachment and spontaneous partitioning in the biodegradation of naphthalene initially present in nonaqueous-phase liquids
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DOI：
10.1128/aem.60.7.2643-2646.1994
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发表时间：
1994-07
影响因子：
4.4
通讯作者：
J. Ortega-Calvo;Martin Alexander
中科院分区：
文献类型：
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作者：
J. Ortega-Calvo;Martin Alexander
一种节肢细菌对溶解在邻苯二甲酸二（2-乙基己基）盐中的萘的矿化表现为一个缓慢的阶段，随后是一个快速的阶段。Triton X-100抑制细胞附着，阻止第二阶段的发生。Triton X-100增加了最初存在于2,2,4,4,6,8,8-七甲基壬烷中的萘的矿化程度。在没有微生物的情况下，附着在界面上的细胞矿化芳香烃的速率是分离速率的四倍，但Triton X-100显著降低了这种微生物活性。我们认为，原本存在于非水相液体中的萘的利用可能包括一个由自由悬浮在水相中的细菌进行的分区有限的初始阶段，以及随后由直接存在于非水-液-水界面的细菌影响的更快速的阶段。
The mineralization by an Arthrobacter sp. of naphthalene initially dissolved in di(2-ethylhexyl)phthalate exhibited a slow phase followed by a rapid phase. Triton X-100, which inhibited cell attachment, prevented the onset of the second phase. Triton X-100 increased the extent of mineralization of naphthalene initially present in 2,2,4,4,6,8,8-heptamethylnonane. Cells attached to the interface mineralized the aromatic hydrocarbon at a rate four times higher than the rate of partitioning in the absence of microorganisms, but this microbial activity was markedly reduced by Triton X-100. We suggest that utilization of naphthalene originally present in nonaqueous-phase liquids may involve a partitioning-limited initial stage carried out by bacteria freely suspended in the aqueous phase and a subsequent, more rapid stage effected by bacteria present directly at the nonaqueous-liquid-water interface.