Phenotypic and genetic polymorphisms among human herpesvirus-6 isolates from North American infants.
Phenotypic and genetic polymorphisms among human herpesvirus-6 isolates from North American infants.
复制标题
来自北美婴儿的人类疱疹病毒 6 分离株的表型和遗传多态性。
DOI：
10.1016/0042-6822(92)91240-u
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发表时间：
1992
期刊：
影响因子：
3.7
通讯作者：
Hall,CB
中科院分区：
文献类型：
--
作者：
Dewhurst,S;Chandran,B;McIntyre,K;Schnabel,K;Hall,CB
15个人疱疹病毒-6（HHV-6）分离物，从北美婴儿原发性感染表现为发热或玫瑰疹样疾病，其表型特征的基础上，在体外生长的连续T细胞系和原代人单核细胞，并通过其与单克隆抗体的反应性。所有分离株在原代人脐带血单核细胞中有效复制，但在人细胞系中观察到五种不同的病毒复制模式。发现两个来自婴儿的HHV-6分离株在HSB-2细胞中复制，这与所谓的A组病毒有关，以前只从成人中分离出来。这些相同的分离株也与一组A特异性单克隆抗体反应。病毒分离株的基因组特征，使用良好的特点限制性位点多态性表明，这两个分离株含有A型和B型基因组的混合物，在不同的比例。这些数据表明，并不是所有的HHV-6分离株都可以分为两大类之一，这种HHV-6分离株的分离实际上可能是误导。
Fifteen human herpesvirus-6 (HHV-6) isolates from North American infants with primary infection manifest as febrile or roseola (exanthem subitum) like illnesses were characterized phenotypically on the basis of theirin vitrogrowth in continuous T-cell lines and primary human mononuclear cells and by their reactivity with monoclonal antibodies. All isolates replicated efficiently in primary human cord blood mononuclear cells, but five distinct patterns of viral replication in human cells lines were observed. Two of the HHV-6 isolates from infants were found to replicate in HSB-2 cells, a property associated with so-called group A viruses, which had previously been isolated only from adults. These same isolates also reacted with a panel of A-specific monoclonal antibodies. Genomic characterization of viral isolates using well-characterized restriction site polymorphisms indicated that these two isolates contained a mixture of both A- and B-type genomes, in different proportions. These data suggest that not all HHV-6 isolates can be categorized into one of two broad groups and that such segregation of HHV-6 isolates may in fact be misleading.