Expression of cDNAs encoding mouse cardiac troponin T isoforms: Characterization of a large sample of independent clones
Expression of cDNAs encoding mouse cardiac troponin T isoforms: Characterization of a large sample of independent clones
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DOI：
10.1016/0378-1119(95)00803-9
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发表时间：
1996-02-12
期刊：
影响因子：
3.5
通讯作者：
Zhang, JY
中科院分区：
文献类型：
--
作者：
Jin, JP;Wang, J;Zhang, JY
我们已经分离出52个小鼠心肌肌钙蛋白编码的cDNA克隆（TnT）的特异性抗体筛选的λ ZAP Ⅱ表达文库。从大样本的独立cDNA的测序数据表明，心脏TnT基因的4个选择性剪接外显子的表达之间的关系，产生7类cDNA编码4种蛋白质异构体不同的两个可变区。在N末端可变区和胚胎特异性外显子4旁边，在20%的成人亚型中发现了选择性剪接的外显子3a。选择性剪接的外显子12，对应于一个中央可变区，TnT的两个功能结构域之间的区域，被发现在约。在52个小鼠心肌TnT cDNA中，79%的cDNA具有完全消除内部受体位点处的剪接的单碱基突变。在外显子2的5 '-非翻译区发现了三个不同频率的可变剪接受体位点。
We have isolated 52 mouse cardiac troponin-Tr-encoding cDNA clones (TnT) by specific antibody screening of a lambda ZAPII expression library. Sequencing data from the large sample of independent cDNAs demonstrated relationships among the expression of four alternatively-spliced exons of the cardiac TnT gene, producing seven classes of cDNAs encoding four protein isoforms differing in two variable regions. In the N-terminal variable region and next to the embryonic-specific exon 4, an alternatively spliced exon 3a was identified in 20% of the adult isoforms. The alternatively spliced exon 12, corresponding to a central variable, region between the two functional domains of TnT, was found in approx. 79% of the 52 mouse cardiac TnT cDNAs with a single base mutation completely abolishing the splicing at an internal acceptor site. Three novel alternative splicing acceptor sites in the 5'-untranslated portion of exon 2 have been identified with different frequencies.