CHARACTERIZATION OF HUMAN HIGH-DENSITY LIPOPROTEINS BY GRADIENT GEL-ELECTROPHORESIS
CHARACTERIZATION OF HUMAN HIGH-DENSITY LIPOPROTEINS BY GRADIENT GEL-ELECTROPHORESIS
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DOI：
10.1016/0005-2760(81)90253-8
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
NICHOLS, AV
中科院分区：
文献类型：
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作者：
BLANCHE, PJ;GONG, EL;NICHOLS, AV
梯度凝胶电泳结合自动光密度测定法被应用于鉴定和评估人血浆中超离心d（密度）≤1.200组分中高密度脂蛋白（HDL）的亚群。一组（n = 194）人类受试者的HDL模式中亚群峰相对迁移距离（RF值）的频率分布显示出5个明显的最大值：2个在与HDL2亚类相关的RF范围内，3个在HDL3亚类的RF范围内。在界定这些最大值的RF区间内的HDL被指定为（HDL2b）gge、（HDL2a）gge、（HDL3a）gge、（HDL3b）gge和（HDL3c）gge，并且被证明大致对应于通过分析超速离心在HDL2b、HDL2a和HDL3组分内确定的物质。通过对超速离心施利伦（Schlieren）模式进行三组分分析所解析的由HDL2a组分所代表的物质，经梯度电泳发现其在颗粒大小上是多分散的。与频率最大值的RF值相对应的HDL的平均水合密度和颗粒大小分别为：（HDL2b）gge中为1.085 g/ml和10.57 nm；（HDL2a）gge中为1.115 g/ml和9.16 nm；（HDL3a）gge中为1.136 g/ml和8.44 nm；（HDL3b）gge中为1.154 g/ml和7.97 nm；（HDL3c）gge中为1.171 g/ml和7.62 nm。（HDL3a）gge和（HDL3b）gge内的亚群的平均水合密度值与最近通过区带超速离心所表征的HDL3L和HDL3D组分的那些值相当。从梯度凝胶获得的（HDL2b）gge、（HDL2a）gge和（HDL3）gge物质的光密度扫描与从分析超速离心获得的HDL2b、HDL2a和HDL3组分的血浆浓度之间显示出高阶且具有统计学意义的相关性。
Gradient gel electrophoresis in conjunction with automated densitometry was applied to the identification and estimation of subpopulations of high-density lipoproteins (HDL) in the ultracentrifugal d [density] .ltoreq. 1.200 fraction from human plasma. The frequency distribution of relative migration distances (RF values) of subpopulation peaks in HDL patterns of a group (n = 194) of human subjects showed 5 apparent maxima: 2 in the RF range associated with the HDL2 subclass, and 3 in the RF range of the HDL3 subclass. HDL within RF intervals bounding these maxima were designated (HDL2b)gge, (HDL2a)gge, (HDL3a)gge, and (HDL3b)gge and (HDL3c)gge and were shown to correspond approximately to material determined by analytic ultracentrifugation within the HDL2b, HDL2a and HDL3 components. Material represented by the HDL2a component, as resolved by 3-component analysis of the ultracentrifugal Schlieren pattern, was found by gradient electrophoresis to be polydisperse in particle size. Mean hydrated densities and particle sizes of HDL corresponding to those with RF values of the frequency maxima were: 1.085 g/ml and 10.57 nm in the (HDL2b)gge; 1.115 g/ml and 9.16 nm in the (HDL2a)gge; 1.136 g/ml and 8.44 nm in the (HDL3a)gge; 1.154 g/ml and 7.97 nm in the (HDL3b)gge; and 1.171 g/ml and 7.62 nm in the (HDL3c)gge. The mean hydrated density values of the subpopulations within the (HDL3a)gge and (HDL3b)gge were comparable to those of the HDL3L and HDL3D components recently characterized by zonal ultracentrifugation. High order and statistically significant correlations between densitometric scans of the (HDL2b)gge, (HDL2a)gge and (HDL3)gge material, as obtained from gradient gels, and plasma concentrations of the HDL2b, HDL2a and HDL3 components, as obtained from analytic ultracentrifugation, were demonstrated.