A novel membrane based process to isolate photosystem-I membrane complex from spinach
A novel membrane based process to isolate photosystem-I membrane complex from spinach
复制标题
一种从菠菜中分离光系统-I膜复合物的新型膜工艺
DOI：
10.1007/s11120-011-9625-5
复制
发表时间：
2011-01
影响因子：
3.7
通讯作者：
Zhanfeng Cui
中科院分区：
文献类型：
--
作者：
Jianguo Liu;Mengmen Yin;Meng Wang;Xuefang Zhang;Baosheng Ge;Shuang Liu;Jianren Lu;Zhanfeng Cui
The isolation of photosystem-I (PS-I) from spinach has been conducted using ultrafiltration with 300 kDa molecular weight cut-off polyethersulfone membranes. The effects of ultrafiltration operating conditions on PS-I activity were optimized using parameter scanning ultrafiltration. These conditions included solution pH, ionic strength, stirring speed, and permeate flux. The effects of detergent (Triton X-100 andn-dodecyl-beta-D-maltoside) concentration on time dependent activity of PS-I were also studied using an O2electrode. Under optimized conditions, the PS-I purity obtained in the retentate was about 84% and the activity recovery was greater than 94% after ultrafiltration. To our knowledge, this is the first report of the isolation of a membrane protein using ultrafiltration alone.
登录
查看更多内容
DOI：
10.1007/s00249-009-0553-7
发表时间：
2010-03
期刊：
European Biophysics Journal
影响因子：
--
作者：
W. Vos;Sebastian Vaughan;P. Lall;J. McCaffrey;Monika Wysocka-Kapcińska;J. Findlay
通讯作者：
W. Vos;Sebastian Vaughan;P. Lall;J. McCaffrey;Monika Wysocka-Kapcińska;J. Findlay
设计两亲性短肽增强孤立光系统-I 的热稳定性
影响因子：
3.7
作者：
Ge B;Yang F;Yu D;Liu S;Xu H
通讯作者：
Xu H
影响因子：
3.7
作者：
E. Šetlíková;S. Ritter;R. Hienerwadel;J. Kopecký;J. Komenda;W. Welte;I. Šetlík
通讯作者：
E. Šetlíková;S. Ritter;R. Hienerwadel;J. Kopecký;J. Komenda;W. Welte;I. Šetlík
影响因子：
4.9
作者：
K. Satoh
通讯作者：
K. Satoh
影响因子：
2.9
作者：
Minai, L;Fish, A;Nechushtai, R
通讯作者：
Nechushtai, R