Airway contractility in the precision-cut lung slice after cryopreservation.
Airway contractility in the precision-cut lung slice after cryopreservation.
复制标题
冷冻保存后精密切割肺切片的气道收缩力。
DOI：
10.1165/rcmb.2013-0166ma
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发表时间：
2014
影响因子：
6.4
通讯作者：
Krishnan,Ramaswamy
中科院分区：
文献类型：
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作者：
Rosner,SoniaR;Ram-Mohan,Sumati;Paez-Cortez,JesusR;Lavoie,TeraL;Dowell,MariaL;Yuan,Lei;Ai,Xingbin;Fine,Alan;Aird,WilliamC;Solway,Julian;Fredberg,JeffreyJ;Krishnan,Ramaswamy
气道生物学中的一种新兴工具是精确切割肺切片（PCLS）。采用PCLS作为评估气道反应性的模型受到组织保持活力的有限时间窗的阻碍。在这里，我们证明了PCLS可以冷冻，长期储存，然后解冻供以后的实验使用。与从未冷冻的小鼠PCLS相比，冷冻-解冻的PCLS显示代谢活性降低到与在其他冷冻保存的组织中观察到的相当的程度，但在细胞活力或对收缩激动剂乙酰甲胆碱或舒张激动剂氯喹的气道口径反应方面没有差异。这些结果表明，冷冻和长期储存是一个可行的解决问题的有限的生活力的PCLS在培养。
An emerging tool in airway biology is the precision-cut lung slice (PCLS). Adoption of the PCLS as a model for assessing airway reactivity has been hampered by the limited time window within which tissues remain viable. Here we demonstrate that the PCLS can be frozen, stored long-term, and then thawed for later experimental use. Compared with the never-frozen murine PCLS, the frozen-thawed PCLS shows metabolic activity that is decreased to an extent comparable to that observed in other cryopreserved tissues but shows no differences in cell viability or in airway caliber responses to the contractile agonist methacholine or the relaxing agonist chloroquine. These results indicate that freezing and long-term storage is a feasible solution to the problem of limited viability of the PCLS in culture.