Counting chromosomes in intact eggs.
Counting chromosomes in intact eggs.
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DOI：
10.1007/978-1-62703-191-2_17
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发表时间：
2013-01-01
期刊：
影响因子：
--
通讯作者：
Lampson, Michael A
中科院分区：
文献类型：
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作者：
Chiang, Teresa;Lampson, Michael A
染色体扩散是评估不同细胞类型中倍性的既定方法。然而，许多传统的染色体扩散技术需要溶解细胞，并且由于该过程中固有的潜在染色体丢失，因此只能用于评估超倍性。在这里，我们描述了一种方法，以评估染色体数目在完整的鸡蛋，使超倍性和亚倍性可以准确地检测。
Chromosomal spreads are an established method to assess ploidy in different cell types. However, many traditional chromosome-spreading techniques require dissolution of the cell and can only be used to assess hyperploidy because of potential chromosome loss inherent in the procedure. Here we describe a method to evaluate chromosome numbers in intact eggs so that both hyperploidy and hypoploidy can be accurately detected.