Binding of APC to the human homolog of the Drosophila discs large tumor suppressor protein
Binding of APC to the human homolog of the Drosophila discs large tumor suppressor protein
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DOI：
10.1126/science.272.5264.1020
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发表时间：
1996-05-17
期刊：
影响因子：
56.9
通讯作者：
Akiyama, T
中科院分区：
文献类型：
--
作者：
Matsumine, A;Ogai, A;Akiyama, T
家族性腺瘤性息肉病和散发性结直肠肿瘤中的腺瘤性息肉病基因（APC）发生突变，其产物与粘附连接蛋白β-连环蛋白结合。APC的过表达阻断细胞周期进程，APC-β-catenin复合物显示与DLG（果蝇盘大肿瘤抑制蛋白的人类同源物）结合，这种相互作用需要APC的羧基末端区域和DLG的DLG同源重复区域。APC与DLG共定位于大鼠结肠上皮细胞的外侧胞质和培养的海马神经元的突触。这些结果表明APC-DLG复合物可能参与细胞周期进程和神经元功能的调节。
The adenomatous polyposis coli gene (APC) is mutated in familial adenomatous polyposis and in sporadic colorectal tumors, and its product binds to the adherens junction protein beta-catenin. Overexpression of APC blocks cell cycle progression, The APC-beta-catenin complex was shown to bind to DLG, the human homolog of the Drosophila discs large tumor suppressor protein, This interaction required the carboxyl-terminal region of APC and the DLG homology repeat region of DLG. APC colocalized with DLG at the lateral cytoplasm in rat colon epithelial cells and at the synapse in cultured hippocampal neurons. These results suggest that the APC-DLG complex may participate in regulation of both cell cycle progression and neuronal function.