The RXL motif of the African cassava mosaic virus Rep protein is necessary for rereplication of yeast DNA and viral infection in plants.
The RXL motif of the African cassava mosaic virus Rep protein is necessary for rereplication of yeast DNA and viral infection in plants.
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DOI：
10.1016/j.virol.2014.06.002
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发表时间：
2014-08
期刊：
影响因子：
3.7
通讯作者：
K. Hipp;P. Rau;Benjamin Schäfer;B. Gronenborn;H. Jeske
中科院分区：
文献类型：
--
作者：
K. Hipp;P. Rau;Benjamin Schäfer;B. Gronenborn;H. Jeske
双链病毒，单链DNA植物病毒，编码复制启动蛋白（Rep），这是病毒复制不可或缺的。非洲木薯花叶病毒Rep序列中潜在的周期蛋白相互作用基序（RXL）可能是细胞周期控制与已知的植物视网膜母细胞瘤蛋白（pRBR）同源物相互作用的另一种联系。该基序的突变消除了野生型Rep诱导的裂变酵母的rereplication，这表明Rep通过其RXL基序与一种或几种酵母蛋白相互作用。RXL基序对于benthamiannicotiana的病毒感染至关重要，因为该基序在感染性克隆中的突变可以防止任何症状性感染。研究了一种纳米病毒（蚕豆坏死黄病毒）的细胞周期链接（Clink）蛋白，该蛋白通过其LXCXE基序与pRBR结合激活细胞周期。在裂变酵母菌中，野生型Clink和缺乏prbr结合的Clink突变体的表达没有触发rereplication。
Geminiviruses, single-stranded DNA plant viruses, encode a replication-initiator protein (Rep) that is indispensable for virus replication. A potential cyclin interaction motif (RXL) in the sequence of African cassava mosaic virus Rep may be an alternative link to cell cycle controls to the known interaction with plant homologs of retinoblastoma protein (pRBR). Mutation of this motif abrogated rereplication in fission yeast induced by expression of wildtype Rep suggesting that Rep interacts via its RXL motif with one or several yeast proteins. The RXL motif is essential for viral infection ofNicotiana benthamianaplants, since mutation of this motif in infectious clones prevented any symptomatic infection. The cell-cycle link (Clink) protein of a nanovirus (faba bean necrotic yellows virus) was investigated that activates the cell cycle by binding via its LXCXE motif to pRBR. Expression of wildtype Clink and a Clink mutant deficient in pRBR-binding did not trigger rereplication in fission yeast.