Establishing an environmental DNA method to detect and estimate the biomass of Sakhalin taimen, a critically endangered Asian salmonid
Establishing an environmental DNA method to detect and estimate the biomass of Sakhalin taimen, a critically endangered Asian salmonid
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DOI：
10.1007/s10201-017-0535-x
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发表时间：
2018-04-01
期刊：
影响因子：
1.6
通讯作者：
Araki, Hitoshi
中科院分区：
文献类型：
--
作者：
Mizumoto, Hiroki;Urabe, Hirokazu;Araki, Hitoshi
对于野外生态学家来说，在野外检测目标物种是了解其分布和种群状况的严重瓶颈。近年来，环境DNA（eDNA）技术已发展成为一种非侵入性的水生生物监测工具。虽然已经提出了eDNA技术的生物量估计的应用程序，鲜为人知的是一个适用的大小范围的生物体，这可能会影响生物量和eDNA浓度之间的关系。在这里，我们调查了来自日本北方的一种巨大的鲑鱼科物种--库页岛哲罗鱼（Parahuchoperryi）的eDNA。该物种是极度濒危物种，很难通过传统的采样方法在野外发现。使用定量实时PCR，我们测试了eDNA浓度和鱼类密度之间的相关性，使用不同年龄和身体大小的鱼在水族馆实验。我们发现我们的新引物和探针是真正的种特异性，并且eDNA浓度与鱼类密度和体型显著相关（p < 0.001）。此外，根据我们的计算，eDNA的浓度在水族馆与不同年龄和大小组的鱼，当他们的总重量调整后，相当恒定。这些结果表明，eDNA浓度可以作为库页岛哲罗鱼生物量的一个指标，但其在自然环境中的应用还需要进一步的研究。
For field ecologists, detecting a target species in the wild is a severe bottleneck to understanding its distribution and population status. Recently, environmental DNA (eDNA) techniques have been developed as a noninvasive monitoring tool for aquatic organisms. While applications of eDNA techniques for biomass estimation have been proposed, little is known about an applicable size range of the organisms, which might affect relationships between biomass and eDNA concentration. Here, we investigated eDNA from Sakhalin taimen (Parahucho perryi), a giant salmonid species of northern Japan. This species is critically endangered and difficult to detect in the wild by conventional sampling methods. Using quantitative real-time PCR, we tested correlations between eDNA concentration and fish density using fish with a wide range of ages and body sizes in aquarium experiments. We found that our new primers and probe were truly species-specific, and that the eDNA concentration was significantly correlated with fish density and body size (p < 0.001). Furthermore, based on our calculation, the eDNA concentrations were rather constant among aquaria with fish in different age and size groups when their total weight was adjusted. These results suggest that eDNA concentrations can be an indicator of biomass of Sakhalin taimen, although further research is needed for its application in natural environments.