Engrailed-1 as a target of the Wnt-1 signalling pathway in vertebrate midbrain development
Engrailed-1 as a target of the Wnt-1 signalling pathway in vertebrate midbrain development
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DOI：
10.1038/383332a0
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发表时间：
1996-09-26
期刊：
影响因子：
64.8
通讯作者：
McMahon, AP
中科院分区：
文献类型：
--
作者：
Danielian, PS;McMahon, AP
由Wnt-1基因编码的分泌信号分子是中枢神经系统发育所必需的。在Wnt-1-null等位基因纯合的小鼠胚胎中，中脑和前后脑不能发育（1,2）。这对应于小鼠嵌入基因（En-1和En-2）编码的两个转录因子通常表达的区域（3,4）。对果蝇Wnt-1的同源基因无翅的研究表明，无翅信号是维持发育中的表皮邻近细胞的纠缠表达所必需的（5-7）。我们报道了在Wnt-1缺失胚胎发育中的中脑中En-1的表达足以挽救早期中脑和前后脑的发育。这表明Wnt-1信号传导的一个关键作用是维持En的表达，并且Wnt-1/ engriled相互作用的这一方面从果蝇到小鼠都是保守的。
A SECRETED Signalling molecule encoded by the Wnt-1 gene is required for development of the central nervous system. In mouse embryos homozygous for a Wnt-1-null allele, the midbrain and anterior hindbrain fail to develop(1,2). This corresponds to the region where two transcription factors encoded by the mouse engrailed genes (En-1 and En-2) are normally expressed(3,4). Studies of the Drosophila orthologue of Wnt-1, wingless, indicate that Wingless signal is required to maintain engrailed expression in neighbouring cells of the developing epidermis(5-7). Here we report that expression of En-1 in the developing midbrain of Wnt-1 null embryos is sufficient to rescue early midbrain and anterior hindbrain development. This suggests that a key role of Wnt-1 signalling is to maintain En expression and that this aspect of the Wnt-1/engrailed interaction has been conserved from flies to mice.