Transepithelial transport of flavanone in intestinal Caco-2 cell monolayers
Transepithelial transport of flavanone in intestinal Caco-2 cell monolayers
复制标题
DOI：
10.1016/j.bbrc.2007.12.185
复制
发表时间：
2008-03-28
影响因子：
3.1
通讯作者：
Konishi, Yutaka
中科院分区：
文献类型：
--
作者：
Kobayashi, Shoko;Konishi, Yutaka
我们最近的研究[S.小林、S.田边，M. Sugiyama，Y.小西，橙皮素和橙皮苷在肠Caco-2细胞单层中的跨上皮转运，Biochim. Biophys. Acta，1778（2008）33-41]显示橙皮素的吸收机制涉及质子偶联主动转运和跨细胞被动扩散。在这里，以及分析橙皮素的细胞渗透性，我们还研究了其他黄烷酮，柚皮素和圣草酚的运输，使用Caco-2细胞单层。类似于所提及的橙皮素，柚皮素和圣草酚显示出以非饱和方式在顶部至基底外侧方向上的质子偶联极化转运，在顶部至基底外侧方向上的恒定渗透（J（ap -> bl））而不考虑跨上皮电阻（TER），并且在质子梯度存在下在顶部加载后优选分布到基底外侧中。此外，橙皮素、柚皮素和圣草酚的质子偶联J（ap -> bl）被单羧酸转运蛋白（NICT）的底物如苯甲酸抑制，但不被阿魏酸抑制。相反，通过反式刺激分析，苯甲酸和阿魏酸对每个黄烷酮的J（ap -> bl）没有刺激作用。这些结果表明，质子驱动的主动转运通常参与了黄烷酮的吸收，并且推测其转运是唯一的，而不是MCT介导的酚酸（PA）吸收转运、钠依赖性MCT（SMCT）或阴离子交换剂介导的转运。(C)2008年爱思唯尔公司All rights reserved.
Our recent study [S. Kobayashi, S. Tanabe, M. Sugiyama, Y. Konishi, Transepithelial transport of hesperetin and hesperidin in intestinal Caco-2 cell monolayers, Biochim. Biophys. Acta, 1778 (2008) 33-41] shows that the mechanism of absorption of hesperetin involves both proton-coupled active transport and transcellular passive diffusion. Here, as well as analyzing the cell permeability of hesperetin, we also study the transport of other flavanones, naringenin and eriodictyol, using Caco-2 cell monolayers. Similar to hesperetin mentioned, naringenin and eriodictyol showed proton-coupled polarized transport in apical-to-basolateral direction in non-saturable manner, constant permeation in the apical-to-basolateral direction (J(ap -> bl)) irrespective of the transepithelial electrical resistance (TER), and preferable distribution into the basolateral side after apical loading in the presence of a proton gradient. Furthermore, the proton-coupled J(ap -> bl) of hesperetin, naringenin and eriodictyol, were inhibited by substrates of the monocarboxylic acid transporter (NICT), such as benzoic acid, but not by ferulic acid. In contrast, both benzoic and ferulic acids have no stimulatory effect on J(ap -> bl) of each flavanone by trans-stimulation analysis. These results indicates that proton-driven active transport is commonly participated in the absorption of flavanone in general, and that its transport is presumed to be unique other than MCT-mediated transport for absorption of phenolic acids (PAs), sodium-dependent MCT (SMCT) nor anion exchanger-mediated transport. (C) 2008 Elsevier Inc. All rights reserved.