Biomedical soft contact-lens sensor for in situ ocular biomonitoring of tear contents
Biomedical soft contact-lens sensor for in situ ocular biomonitoring of tear contents
复制标题
DOI：
10.1007/s10544-011-9530-x
复制
发表时间：
2011-08-01
影响因子：
2.8
通讯作者：
Mitsubayashi, Kohji
中科院分区：
文献类型：
--
作者：
Chu, MingXing;Shirai, Takayuki;Mitsubayashi, Kohji
研制了一种用于泪液非侵入性生物监测的软接触透镜生物传感器。在眼睛上佩戴生物传感器能够原位监测泪液含量。该生物传感器在聚二甲基硅氧烷（PDMS）接触透镜的表面上具有酶固定化电极。SCL-生物传感器的功能聚合物（PDMS和2-甲基丙烯酰氧乙基磷酰胆碱（MPC）聚合物）的微加工技术制造。在体外特性的调查，SCL-生物传感器的输出电流和葡萄糖浓度从0.03至5.0 mmol中心点L-1之间表现出良好的关系，相关系数为0.994。校准范围涵盖报告的泪液葡萄糖浓度（0.14 mmol中心点L-1）。在此基础上，用SCL-生物传感器进行了眼部生物监测。SCL-生物传感器在静态和动态下都能很好地工作。首先测得兔泪液葡萄糖浓度为0.12mmol·L ~（-1），然后成功地计算出泪液周转率为29.6A +/-8.42%min ~（-1）。结果表明，SCL-生物传感器是有用的先进的生物监测眼睛。
A soft contact-lens biosensor (SCL-biosensor) for novel non-invasive biomonitoring of tear fluids was fabricated and tested. Wearing a biosensor on eye enabled the in situ monitoring of tear contents. The biosensor has an enzyme immobilized electrode on the surface of a polydimethyl siloxane (PDMS) contact lens. The SCL-biosensor was fabricated using microfabrication techniques for functional polymers (PDMS and 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer). In investigation of in vitro characterization, the SCL-biosensor showed excellent relationship between the output current and glucose concentration from 0.03 to 5.0 mmol center dot L-1, with a correlation coefficient of 0.994. The calibration range covered the reported tear glucose concentrations (0.14 mmol center dot L-1). Based on the result, ocular biomonitoring with the SCL-biosensor was carried out. The SCL-biosensor well worked both in the static state and the dynamic state. The tear glucose level of rabbit was estimated to 0.12 mmol center dot L-1 at first and then the tear turnover was successfully calculated to be 29.6 A +/- 8.42% min(-1). The result indicated that SCL-biosensor is useful for advanced biomonitoring on eye.