The immune responses and antioxidant status of Portunus trituberculatus individuals with different body weights
The immune responses and antioxidant status of Portunus trituberculatus individuals with different body weights
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不同体重三疣梭子蟹个体的免疫反应和抗氧化状态
DOI：
10.1016/j.fsi.2016.03.004
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发表时间：
2016
影响因子：
4.7
通讯作者：
Liu Ping
中科院分区：
文献类型：
--
作者：
Ren Xianyun;Yu Xuan;Gao Baoquan;Li Jian;Liu Ping
溶藻弧菌是一种影响螃蟹水产养殖的剧毒病原体。本文研究了三疣梭子蟹80、100和120日龄的大、小(按体重和大小)标本在溶藻弧菌攻击72小时后的免疫反应和抗氧化状态。大个体(BIS)的血细胞总数(THC)、吞噬功能、酚氧化酶原和酚氧化酶活性均高于小个体(P<；P<0.05)。生物体的抗氧化状态表现出相似的模式：BIS的无细胞血淋巴和肝胰腺中的超氧化物歧化酶(SOD)活性和谷胱甘肽/氧化谷胱甘肽(GSH/GSSG)活性高于SIS(P<；P<；0.05)。α2-巨球蛋白(α2-M)、无细胞血淋巴中的抗菌和溶菌活性以及无细胞血淋巴或肝胰腺中的谷胱甘肽过氧化物酶活性在两组间无显著差异。α2-M和crustin基因在血细胞中的表达水平，以及在血细胞和肝胰腺中的表达水平也显著高于对照组。结果表明，与SIS相比，BIS具有更强的抗藻毒素感染能力。
Vibrio alginolyticusis a virulent pathogen that affects crab aquacultures. In the present study, the immune responses and antioxidant status of big and small (based on body weight and size) 80-, 100- and 120-day-old specimens ofPortunus trituberculatus, challenged for 72 h withVibrio alginolyticus, were studied. The total hemocyte count (THC), and phagocytic, prophenoloxidase and phenoloxidase activities, of the big individuals (BIs) were higher than those of the small individuals (SIs) (P< 0.05). The antioxidant status of the organisms showed a similar pattern: superoxide dismutase (SOD) activity and glutathione/oxidized glutathione (GSH/GSSG) in the cell-free hemolymph and hepatopancreases of the BIs were higher than in the SIs (P< 0.05). There were no significant differences in α2-macroglobulin (α2-M), antibacterial and bacteriolytic activities in the cell-free hemolymph, or glutathione peroxidase activity in the cell-free hemolymph or hepatopancreas between the BIs and SIs. The α2-M and crustin gene expression levels in the hemocytes, and SOD expression in the hemocytes and hepatopancreas, were also significantly higher in the BIs. The results suggest that, compared with the SIs, the BIs possessed a higher resistance toV. alginolyticusinfection.