RENAL CARCINOGENESIS IN THE EKER RAT
RENAL CARCINOGENESIS IN THE EKER RAT
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DOI：
10.1007/bf01197777
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发表时间：
1995-09-01
影响因子：
3.6
通讯作者：
MITANI, H
中科院分区：
文献类型：
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作者：
HINO, O;KOBAYASHI, E;MITANI, H
在实验动物中，Eker大鼠遗传性肾癌是孟德尔显性易感性对特定癌症的一个极好的例子。我们最近报道了人类结节性硬化症（TSC2）基因的大鼠同源物的种系插入在Eker大鼠模型中引起显性遗传性癌症。TSC2/ TSC2基因产物（在人类病例中称为“tuberine”）的功能尚不清楚，尽管它含有与ms家族gtpase激活蛋白（GAP3）同源的短氨基酸序列。在这项研究中，我们分离了Eker肾癌细胞中表达增加的缺失cDNA克隆，使用改进的代表性差异分析方法来寻找特异性参与肾癌发生的其他基因。在这里，我们确定了四个基因：补体（C3）基因的第三部分，fos相关抗原1 （fra-1）基因，一个未知基因（指定在肾癌中表达：erc）和calpactine I重链（Annexin II）基因。
The Eker rat hereditary renal carcinoma is an excellent example of a Mendelian dominant predisposition to a specific cancer in an experimental animal. We recently reported that a germline insertion in the rat homologue of the human tuberous sclerosis (TSC2) gene gives rise to the dominantly inherited cancer in the Eker rat model. The function of the TSC2/Tsc2 gene product (called ''tuberine'' in the human case) is not yet understood, although it contains a short amino acid sequence homologous to the ms family GTPase-activating proteins (GAP3). In the study, we isolated subtracted cDNA clones having increased expression in Eker renal carcinoma cells, using a modified representational difference analysis method to search for additional genes specifically involved in renal carcinogenesis. Here we identified four genes: the third component of the complement (C3) gene, the fos-related antigen 1 (fra-1) gene, an unknown gene (designated as being expressed in renal carcinoma: erc) and the calpactine I heavy-chain (Annexin II) gene.