VESTIBULAR SHORT LATENCY RESPONSES TO PULSED LINEAR ACCELERATION IN UNANESTHETIZED ANIMALS
VESTIBULAR SHORT LATENCY RESPONSES TO PULSED LINEAR ACCELERATION IN UNANESTHETIZED ANIMALS
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DOI：
10.1016/0013-4694(92)90007-5
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发表时间：
1992-05-01
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影响因子：
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通讯作者：
JONES, TA
中科院分区：
文献类型：
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作者：
JONES, TA
首次使用线性加速度瞬变在非侵入性制备的未麻醉动物（小鸡，鸡，n = 33）中引发前庭复合动作电位。 反应由一系列的高达8个主峰发生在8毫秒的刺激。 1.0 g刺激的反应幅度范围从1到10 μ V。一个迟，慢，三相，麻醉不稳定的组成部分被确定为在未麻醉动物的主要反应特征。 随着刺激强度的增加，波幅增加，波幅降低（MANOVA P < 0.05）。 线性回归斜率范围为：振幅= 1.0-5.0 μ-V/g;振幅= -300至-1100 μ-sec/g。 单极性刺激（0.035 ± 0.022 g，n = 11）的平均肌张力显著低于交替极性刺激（0.074 ± 0.028 g，n = 18，P < 0.001）。 双侧耳蜗切除术消除了反应，而双侧耳蜗摘除术没有显着改变反应阈值。 强烈的声掩蔽（100/104 dB SL）对2只动物没有影响，但对其他7只动物的反应幅度产生了小到中等的影响。 变化归因于对前庭终末器官的影响。 单侧迷路阻滞（河豚毒素）的结果表明，P1和N1优先反映同侧第八神经复合动作电位，而超过约2毫秒的组件反映前庭神经元的活动，依赖于两个神经元。 结果表明，短潜伏期前庭复合动作电位可以在未麻醉，非侵入性准备的动物。
Linear acceleration transients were used to elicit vestibular compound action potentials in non-invasively prepared, unanesthetized animals for the first time (chicks, Gallus domesticus, n = 33). Responses were composed of a series of up to 8 dominant peaks occurring within 8 msec of the stimulus. Response amplitudes for 1.0 g stimulus ranged from 1 to 10-mu-V. A late, slow, triphasic, anesthesia-labile component was identified as a dominant response feature in unanesthetized animals. Amplitudes increased and latencies decreased as stimulus intensity was increased (MANOVA P < 0.05). Linear regression slope ranges were: amplitudes = 1.0-5.0-mu-V/g; latencies = -300 to -1100-mu-sec/g. Thresholds for single polarity stimuli (0.035 +/- 0.022 g, n = 11) were significantly lower than those of alternating polarity (0.074 +/- 0.028 g, n = 18, P < 0.001). Bilateral labyrinthectomy eliminated responses whereas bilateral extirpation of cochleae did not significantly change response thresholds. Intense acoustic masking (100/104 dB SL) produced no effect in 2 animals, but did produce small to moderate effects on response amplitudes in 7 others. Changes were attributed to effects on vestibular end organs. Results of unilateral labyrinth blockade (tetrodotoxin) suggest that P1 and N1 preferentially reflect ipsilateral eighth nerve compound action potentials whereas components beyond approximately 2 msec reflect activity from vestibular neurons that depend on both labyrinths. The results demonstrate that short latency vestibular compound action potentials can be measured in unanesthetized, non-invasively prepared animals.