A NEW COLORIMETRIC METHOD FOR THE DETERMINATION OF FREE FATTY-ACIDS WITH ACYL-COA SYNTHETASE AND ACYL-COA OXIDASE
A NEW COLORIMETRIC METHOD FOR THE DETERMINATION OF FREE FATTY-ACIDS WITH ACYL-COA SYNTHETASE AND ACYL-COA OXIDASE
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DOI：
10.1093/oxfordjournals.jbchem.a133380
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发表时间：
1981-01-01
影响因子：
2.7
通讯作者：
KAWAGUCHI, A
中科院分区：
文献类型：
--
作者：
HOSAKA, K;KIKUCHI, T;KAWAGUCHI, A
A rapid and sensitive spectrophotometric assay for free fatty acids using acyl-CoA synthetase and acyl-CoA oxidase is described. It is sensitive to as low as 5 nmol of free fatty acids, and the standard curve is linear up to 100 nmol. The assay consists of the measurement of H2O2 produced from free fatty acids by acyl-CoA synthetase and acyl-CoA oxidase. The quantity of H2O2 is determined by the absorbance at 550 nm in the presence of catalase and 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole. This method shows a broad specificity to long-chain fatty acids and the recoveries of added fatty acids (C12-C18) are more than 90%. The presence or absence of serum components or Escherichia coli cell-free extracts has no significant effect on the recovery of added palmitic acid.
