DIFFERENTIAL EXPRESSION OF SYNAPTIC VESICLE PROTEIN-2 (SV2) ISOFORMS
DIFFERENTIAL EXPRESSION OF SYNAPTIC VESICLE PROTEIN-2 (SV2) ISOFORMS
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DOI：
10.1523/jneurosci.14-09-05223.1994
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发表时间：
1994-09-01
影响因子：
5.3
通讯作者：
SCHELLER, RH
中科院分区：
文献类型：
--
作者：
BAJJALIEH, SM;FRANTZ, GD;SCHELLER, RH
突触囊泡蛋白SV 2A和SV 2B（SV 2 =突触囊泡蛋白2）是属于转运蛋白家族的两种高度相关的蛋白。作为确定SV 2蛋白功能的第一步，我们通过原位杂交、免疫组织化学和同种型特异性抗体免疫沉淀法研究了SV 2A和SV 2B在大鼠脑中的表达。这些分析显示，一种亚型SV 2A在整个大脑中以不同的水平普遍表达。另一种亚型SV 2B的分布更有限，与SV 2A的共表达程度不同。用同种型特异性抗体对脑突触囊泡进行免疫沉淀，然后进行Western分析，表明这两种同种型可以存在于同一突触囊泡上。SV 2蛋白的表达与神经递质表型或其他突触囊泡蛋白亚型的表达均不相关。在发育过程中观察到SV 2B表达发生变化;它在未成熟的大脑中更广泛地表达，并且在尚未建立突触接触的细胞中发现。SV 2的普遍存在和重叠表达表明它们执行所有突触囊泡共有的功能。SV 2亚型的可变和变化的共表达可能表明SV 2功能受突触囊泡的亚型组成的调节。观察到突触囊泡蛋白，都发生在多种亚型，相对于彼此差异表达，表明多达90种不同的囊泡类型是可能的。
The synaptic vesicle proteins SV2A and SV2B (SV2 = synaptic vesicle protein 2) are two highly related proteins belonging to a family of transporters. As a first step toward identifying the function of the SV2 proteins, we examined the expression of SV2A and SV2B in the rat brain by in situ hybridization, immunohistochemistry, and immunoprecipitation with isoform-specific antibodies. These analyses revealed that one isoform, SV2A, is expressed ubiquitously throughout the brain at varying levels. The other isoform, SV2B, has a more limited distribution with varying degrees of coexpression with SV2A. Immunoprecipitation of brain synaptic vesicles with isoform-specific antibodies followed by Western analyses suggests that both isoforms can be present on the same synaptic vesicle. The expression of the SV2 proteins did not correlate either with neurotransmitter phenotype or with the expression of other synaptic vesicle protein isoforms. SV2B expression was observed to change during development; it is more widely expressed in the immature brain and is found in cells that have yet to establish synaptic contacts. The ubiquitous and overlapping expression of the SV2s suggests that they perform a function common to all synaptic vesicles. Variable and changing coexpression of the SV2 isoforms may indicate that SV2 function is regulated by the isoform composition of synaptic vesicles. The observation that the synaptic vesicle proteins, all occurring in multiple isoforms, are differentially expressed with respect to each other indicates that up to 90 different vesicle types are possible.