Nitric oxide functions in stromal cell-derived factor-1-induced cytoskeleton changes and the migration of Jurkat cells
Nitric oxide functions in stromal cell-derived factor-1-induced cytoskeleton changes and the migration of Jurkat cells
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DOI：
10.3892/ol.2018.9429
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发表时间：
2018-11-01
期刊：
影响因子：
2.9
通讯作者：
Wang, Lan
中科院分区：
文献类型：
--
作者：
Luo, Jixian;Wei, Dan;Wang, Lan
基质细胞衍生因子-1(SDF-1)在多种细胞功能中调节多种细胞信号通路，包括细胞迁移、增殖、存活和血管生成。SDF-1诱导的趋化作用是淋巴细胞迁移的重要步骤。然而，调控SDF-1介导的淋巴细胞迁移的分子机制尚不清楚。一氧化氮(NO)被发现在包括迁移在内的许多信号通路中发挥信号分子的作用。本研究探讨了NO在SDF-1诱导的细胞迁移中的作用及其与Jurkat细胞骨架改变的关系。采用Griess反应和免疫印迹分析方法证实Jurkat细胞通过SDF-1刺激产生NO，并利用一氧化氮合酶抑制剂L-NMMA证实NO参与了SDF-1诱导的细胞骨架重排和聚合。此外，Jurkat细胞的迁移不需要NO。研究表明，NO信号通路在SDF-1诱导的细胞骨架改变和Jurkat细胞迁移中起关键作用。这项工作为深入了解急性淋巴细胞白血病的迁移机制，为急性淋巴细胞白血病的治疗策略提供有效的理论依据。
Stromal cell-derived factor-1 (SDF-1) regulates multiple cell signal pathways in a variety of cellular functions, including cell migration, proliferation, survival and angiogenesis. SDF-1-induced chemotaxis is an important step of lymphocyte migration. However, the molecular mechanisms that modulate SDF-1-mediated lymphocyte migration are not well identified. Nitric oxide (NO) has been found to function as a signaling molecule in a number of signaling pathways, including migration. In the present study, the potential role of NO in SDF-1-induced migration and the association between NO and the cytoskeletal changes of Jurkat cells was investigated. The present study demonstrated that Jurkat cells induced the production of NO by SDF-1 stimulation, using Griess reaction method and western blot analysis, and that NO was involved in SDF-1-induced rearrangement and polymerization of the cytoskeleton, using NOS inhibitor L-NMMA. Furthermore, NO was required for the migration of Jurkat cells. The research suggested that NO signaling pathways exerted a critical role in SDF-1-induced cytoskeleton changes and the migration of Jurkat cells. This work provides insight into the migration mechanism of acute lymphoblastic leukemia and provides an effective theoretical basis for therapy strategies for acute lymphoblastic leukemia.