Dual parallel electrospray ionization and atmospheric pressure chemical ionization mass spectrometry (MS), MS/MS and MS/MS/MS for the analysis of triacylglycerols and triacylglycerol oxidation products
Dual parallel electrospray ionization and atmospheric pressure chemical ionization mass spectrometry (MS), MS/MS and MS/MS/MS for the analysis of triacylglycerols and triacylglycerol oxidation products
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DOI：
10.1002/rcm.581.abs
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发表时间：
2002-01-01
影响因子：
2
通讯作者：
Neff, WE
中科院分区：
文献类型：
--
作者：
Byrdwell, WC;Neff, WE
两个质谱仪，在平行，同时用于分析芥花油中的三酰甘油，用于分析三油酸甘油酯氧化产物，并用于分析使用反相高效液相色谱分离的三酰甘油位置异构体。三重四极杆质谱仪通过大气压化学电离（APCI）接口连接到两个串联的反相液相色谱柱。使用电喷雾电离（ESI）接口将离子阱质谱仪连接到相同的两个柱上，并添加甲酸铵作为电解质。在这些条件下的电喷雾电离质谱（ESI-MS）从三酰基甘油产生丰富的铵加合物离子，然后将其片段化以产生MS/MS谱，然后进一步片段化以产生MS/MS/MS谱。氨化加合物离子的ESI-MS/MS给出了与APCI-MS获得的质谱相似的产物离子质谱。ESI-MS/MS产生了二酰甘油碎片离子，并且额外的碎片化（MS/MS/MS）产生了[RCO]（+）（酰基）离子、[RCOO + 58]（+）离子和其他相关离子，这些离子允许分配各个酰基链身份。三酰甘油氧化产物的APCI-MS产生的光谱与先前使用APCI-MS报道的光谱相似。APCI-MS/MS产生与单个脂肪酸链相关的离子。三酰甘油氧化产物的ESI-MS产生了丰富的铵加合物离子，即使对于那些以前在APCI-MS条件下产生很少或没有完整分子离子的分子。[M+ NH 4]（+）离子的碎片化（MS/MS）产生的结果与APCI-MS获得的结果相似。氧化产物的二酰基甘油片段的进一步碎片化（MS/MS/MS）提供了关于氧化的单个脂肪酰基链的信息。ESI-MS和APCI-MS被认为是互补的技术，它们共同有助于更好地理解由三酰基甘油氧化形成的产物的身份。版权所有（C）2002约翰威利父子有限公司
Two mass spectrometers, in parallel, were employed simultaneously for analysis of triacylglycerols in canola oil, for analysis of triolein oxidation products, and for analysis of triacylglycerol positional isomers separated using reversed-phase high-performance liquid chromatography. A triple quadrupole mass spectrometer was interfaced via an atmospheric pressure chemical ionization (APCI) interface to two reversed-phase liquid chromatographic columns in series. An ion trap mass spectrometer was coupled to the same two columns using an electrospray ionization (ESI) interface, with ammonium formate added as electrolyte. Electrospray ionization mass spectrometry (ESI-MS) under these conditions produced abundant ammonium adduct ions from triacylglycerols, which were then fragmented to produce MS/MS spectra and then fragmented further to produce MS/MS/MS spectra. ESI-MS/MS of the ammoniated adduct ions gave product ion mass spectra which were similar to mass spectra obtained by APCI-MS. ESI-MS/MS produced diacylglycerol fragment ions, and additional fragmentation (MS/MS/MS) produced [RCO](+) (acylium) ions, [RCOO + 58](+) ions, and other related ions which allowed assignment of individual acyl chain identities. APCI-MS of triacylglycerol oxidation products produced spectra like those reported previously using APCI-MS. APCI-MS/MS produced ions related to individual fatty acid chains. ESI-MS of triacylglycerol oxidation products produced abundant ammonium adduct ions, even for those molecules which previously produced little or no intact molecular ions under APCI-MS conditions. Fragmentation (MS/MS) of the [M+NH4](+) ions produced results similar to those obtained by APCI-MS. Further fragmentation (MS/MS/MS) of the diacylglycerol fragments of oxidation products provided information on the oxidized individual fatty acyl chains. ESI-MS and APCI-MS were found to be complementary techniques, which together contributed to a better understanding of the identities of the products formed by oxidation of triacylglycerols. Copyright (C) 2002 John Wiley Sons, Ltd.