ROLLING REPLICATION OF SHORT DNA CIRCLES
ROLLING REPLICATION OF SHORT DNA CIRCLES
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DOI：
10.1073/pnas.92.10.4641
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发表时间：
1995-05-09
影响因子：
11.1
通讯作者：
XU, SQ
中科院分区：
文献类型：
--
作者：
FIRE, A;XU, SQ
天然基因和蛋白质通常含有串联重复的序列基序，这些基序显著增加了生理特异性和活性。考虑到这种重复序列的选择价值，可能有几种不同的机制负责它们的产生。已经显示出产生相对长的串联重复（在内切酶范围内）的一种机制是滚环复制。在该通信中，我们证明了在简单的酶系统中的滚环合成可以产生短至34 bp的单体的串联重复。除了暗示天然串联重复序列的可能起源外，这些观察结果还为构建重复基序文库提供了一种简便的方法，用于“体外进化”实验，旨在选择具有确定生物学或化学特性的分子。
Natural genes and proteins often contain tandemly repeated sequence motifs that dramatically increase physiological specificity and activity. Given the selective value of such repeats, it is likely that several different mechanisms have been responsible for their generation. One mechanism that has been shown to generate relatively long tandem repeats (in the kilobase range) is rolling circle replication, In this communication,,ve demonstrate that rolling circle synthesis in a simple enzymatic system can produce tandem repeats of monomers as short as 34 bp. In addition to suggesting possible origins for natural tandem repeats, these observations provide a facile means for constructing Libraries of repeated motifs for use in ''in vitro evolution'' experiments designed to select molecules with defined biological or chemical properties.